|
With the development of molecular biology, emerging and traditional histological type distinct gene expression profile-based genotyping of breast cancer can reflect the biological characteristics of breast cancer, suggesting that patients prognosis and to provide an effective target for the treatment of . However, genotyping of breast cancer research is still in its infancy, Nielsen diagnostic criteria accepted by most scholars. Genotyping the clinical features of breast cancer there is to be further explored, and its prognostic significance is debatable. The research data come from Western countries, Asia rarely reported domestic only see the South report, yet to be seen in northern China epidemiology, prognosis detailed analysis of the breast cancer population. Objective: In this study, immunohistochemical techniques, cases of invasive breast cancer research by a group of small samples; describe the clinical features and prognosis of basal-like breast cancer in the breast cancer population; 2 by immunohistochemical techniques study to determine basal cell-like breast cancer prognostic factors. Methods: A retrospective analysis of the 2001-2006 Medical School freshman surgery of 953 cases of invasive breast cancer cases, using immunohistochemical method for detection of ER, HER2, CK5 / 6, EGFR expression, by Zhao Nielsen standards, basal cell-like type (Basal-like breast carcinoma, BLBC) 20 cases, select the luminal A (Luminal A) 22 cases, luminal B (Luminal B) 23 cases, HER2 over-expressing (HER2 over-expression) of 23 cases and undivided 17 cases as control, analysis of the clinical features of each type, histological grade and prognosis. 2 Immunohistochemical detection of E-Cadherin five types of breast cancer, MMP-7, Ki-67, P53, caveolin-1 expression, unique expression analysis of basal-like breast cancer characteristics and significance analysis Basaloid prognostic factors in breast cancer. Results: Luminal A-type, Luminal B type, BLBC, HER2 over-expression type and age of onset of unclassified 54.54,51.10,48.80,56.04,51.97 (years) was significantly lower than in BLBC HER2 overexpression (P lt; 0.05), and the rest of the no significant difference (P gt; 0.05); the tumor size of 3.43,3.49,2.12,2.97,3.50 (cm), in BLBC obvious less than LuminalA type, Luminal B type and unclassified (P lt; 0.05). BLBC five types of breast cancer and HER2 overexpression in the clinical stage, lymph node metastasis was no difference, but the BLBC survival 40% (8/12) below the Luminal A type 78.3% (17/22), Luminal B type 73.9 % (17/23, P = 0.0472, P = 0.0452, P lt; 0.05), in BLBC survival rate of less than HE R2 overexpression 65.2% (15/23), not parting 70.6% (12/17), but was not statistically significant (P gt; 0.05) Luminal A.Luminal B.BLBC.HER2 overexpression unclassified the E.Cadherin positive rates were 100% (22/22), 95.7% (22/23), 80% (16/20), 100% (23/23), 94.1% (16/17), in BLBC expression is lower than the other four types (P = 0.017, P lt; 0.05); MMP-7 expression rate was 40.9% (9/22), 52.2% ( 12/23), 85% (17/20), 69.6% (16/23), 64.7% (11/17), BLBC was significantly higher (P = 0.037, P lt; 0.05) The P53 expression rate was 22.7% (5/22), 30.4% (7/23), 60.0% (12/20), 56.5% (13/23), 23.5% (4/17), BLBC.HER2 overexpression type was significantly increased (P 0.021, P lt; 0.01), of Caveolin-1, the expression rate, respectively, to 4.8% (1/21), 13.0% (3/23), 85.0% (17/20), 17.4% (4 / 23), 11.8% (2/17), in BLBC significantly higher than the other four types (P = 0.000, P lt; 0.01) Of Ki-67 expression in BLBC significantly higher than the other four types (P = 0.000, P lt; 0.01). Caveolin-1 expression was 25.7% (27/105), the expression of CK5 / 6 positive rate of 20.6% (20/97), caveolin-1 expression of CK5 / 6, the expression of p53 positive correlation (P lt; 0.01). Conclusion: the BLBC genotyping breast cancer prognosis worst, due to the invasion and metastasis, cell proliferation, proliferation, and prone to mutation of P53 gene. BLBC has the force with E.Cadherin of invasion and metastasis low expression of MMP-7.Caveolin-1 expression. In univariate analysis, the low survival rate of in BLBC E-Cadherin.MMP-7.Caveolin-1.Ki-67, but in the multivariate analysis, E-Cadherin.MMP-7.Caveolin-1.Ki-67 is not a short important independent prognostic factors for survival rate.
|