Dissertation > Excellent graduate degree dissertation topics show

Denervated Skeletal Muscle Atrophy and Experimental Study on Losartan Decreasing Muscle Atrophy Through NF-κB/MuRF1 Pathway

Author: WangLe
Tutor: LiangBingSheng
School: Shanxi Medical
Course: Surgery
Keywords: Muscular atrophy Denervation Losartan NF-κB MuRF1
CLC: R685
Type: Master's thesis
Year: 2010
Downloads: 71
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE:To explore the role of nuclear factor ofκB(NF-κB) and muscle RING finger protein1(MuRF1) in denervated skeletal muscle atrophy in rats and the effect of losartan on it. NF-κB/MuRF1 pathway is one of the most important molecular mechanism in skeletal muscle atrophy. Inhibiting of NF-κB/MuRF1 pathway improves denervated skeletal muscle strength,maintains mass,and promotes regeneration.METHODS:Forty two Wistar rats were randomly divided into 3 groups:Denervated group(n=18). Losartan group (n=18). control group (n=6).The rats were not treated in control group,and were made denervated gastrocnemius models in denervated group and losartan group. In losartan group, the rats were treated with losartan 10mg/kg·d by gavage and with normal saline in denervated group. After 2 day,14day and 28day,levels of NF-κB and MuRF1 mRNA and protein in the gastrocnemius were detected respectively by RT-PCR and Western Bloting. The ratio of muscle wet weight was also analyzed for comparison.RESULTS:Expressions of NF-κB and MuRFl mRNA and protein in denervated skeletal muscle were up-regulated at 2,14,28 days in the early stage of denervation(P<0.05).Moreover, The expressions of NF-κB positively correlated with the expressions of MuRF1(P＜0.05). At 14,28 days after denernation, The losartan group(B) had a higher ratio of muscle wet weight than denervated group(A)(P<0.05). At2,14,28 days after denernation, The expression of NF-kB and MuRF1 in losartan group remarkably reduced comparing with the denervated group (P＜0.05).CONCLUTION:At the early stage of denervated skeletal muscle atrophy in Wistar rats, expressions of NF-κB and MuRF1 are up-regulated, and proved NF-κ/MuRF1 pathway was exist. Losartan can decrease denervated skeletal muscle atrophy through NF-κB/MuRF1 pathway.

Related Dissertations

  1. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  2. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  3. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363
  4. The Molecular Mechanism Intervention of Selenium on the Learning and Memory Injury by Drinking Water Fluorosis,R599
  5. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  6. Effects of Partial Hepatic Ischemia/Reperfusion Injury on Postoperative Cognitive Function in Mice,R614
  7. Experimental Study of Influence of Atrovastatin on Expression of NF-κB in Retionpathy of Diabetic Rats,R587.1
  8. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  9. The Sequence Analysis of RNA Components from Lactobacillus Metabolites and Parts of the Biological Function,R378
  10. Study on Therapy and Mechanisms of the Role of Tang Kang in Diabetic Rat and Endothelial Cells,R285.5
  11. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  12. Effects of Losartan on Na~+, K~+-ATPase in Proximal Tubules Epithelial Cells of Spontaneously Hypertensive Rats,R544.1
  13. Effects of Hypothermia Resuscitation on PPAR-γ and Related Gene Expression in Rats Lung during Hemorrhagic Shock,R605.971
  14. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  15. The Research of Nf-kB、TNF-a and PLGF in the Pathogenesis and Severity of Preeclampsia and Theirs Relevance,R714.244
  16. Restructuring Agkistrodon disintegrin Adinbitor human non-small cell lung cancer cell line A549,R285.5
  17. NF-κB inhibitor PDTC on non-small cell lung cancer cells and JNK protein expression iASPP impact,R734.2
  18. Danxingtongluo decoction on cerebral ischemia-reperfusion injury in TNF-α, NF-κB expression,R285.5
  19. Effects of the Artificial Synthetic E-selectin on the Expression of NF-κB and MCP-1 in an Elastase-induced Model of Common Carotid Artery Aneurysm in Rabbits,R651.1
  20. The Study of Risk Factors and the Correlation of NF-κB and GM-CSF with Bone Metastasis in Breast Cancer,R737.9
  21. The Synthesis of Losartan Derivatives 5-Carbolic Acid Losartan and Inhalation Anesthetics Desflurance,TQ463

CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Muscle disease and injury
© 2012 www.DissertationTopic.Net  Mobile