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Objective: To establish nutritional obesity mouse model, screening of nutritional obese and normal mice in different time periods adipose tissue-specific protein. Methods: C57BL/6J mice 50, a week after the adaptive feeding, were randomly divided into the high-fat group with the low-fat group, respectively, to the high fat diet (58 kcal% fat) and its control low-fat diet (11 kcal% fat) The Feeding 14 weeks. At 0 weeks, 4 weeks, 14 weeks, blood, epididymal adipose tissue, 4 weeks, 11 weeks, 13 weeks, IPGTT. Using protein two-dimensional gel electrophoresis separation of four weeks, 14 weeks of high-fat group and the low-fat group adipose tissue protein, PD-Quest software electrophoresis results do choose to have a significantly different protein spots series media-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-TOF) analysis. Results: high-fat group with the low-fat group weight 1 weekend a significant difference (p lt; 0.05). 4 weeks IPGTT0 points, 15 points, 30 points, 60 points blood sugar showed no statistical significance (p gt; 0.05), high-fat group and the low-fat group 120 points blood glucose difference was statistically significant (p lt; 0.05); high fat group and the low-fat group TG, TG, HDL-C, LDL-C, uric acid difference was statistically significant (p lt; 0.05); fasting venous plasma glucose, insulin difference was not statistically significant (p gt; 0.05). 11 weeks the IPGTT 120 blood glucose difference was statistically significant (p lt; 0.05); 0 minutes, 15 minutes, 30 minutes, 60 minutes of high-fat group and the low-fat group difference was not statistically significant (p gt; 0.05) blood sugar. 13 weeks IPGTT 15 points, 30 points, 120 points of the high-fat group and the low-fat group, glucose difference was statistically significant (p lt; 0.05); 0 minutes, 60 minutes of high-fat group and the low-fat group no significant difference in blood glucose (p gt; 0.05). 14 weeks of high-fat group and the low-fat group TC, TG, HDL-C, LDL-C, uric acid, fasting venous blood glucose difference was statistically significant (p lt; 0.05); Insulin was no significant difference (p gt; 0.05 ). 2D electrophoresis Tip: 14 weeks of high-fat group and the low-fat group difference of no more than 1.5 times, the difference between 1.2-1.5 times 18 points, down 17 points, up one point. 1.5 times more than the 10 point difference in the four weeks of high-fat group and the low-fat group, raised a lowered 9; 26 point difference between 1.2-1.5 times, down 25, up one. We choose a significant difference of 42 points for mass spectrometry analysis. MALDI-TOF-TOF analysis results obtained 16 trusted proteins. Conclusion: The biochemical results suggest that: nutrition obesity mouse model created successfully, the performance of this model with obesity and metabolic syndrome. Four weeks early positioning obesity, there is only abnormal lipids, glucose tolerance, and uric acid, and no impaired fasting glucose and insulin resistance. The model only impose dietary factors alone, the production process is simple and reproducible, short production cycles (13 weeks), and the pathogenesis of human obesity pathogenesis excellent analog obesity early and end late The process of change is a good animal model, and to lay a solid foundation for the follow-up study. May differentially expressed proteins were identified by two-dimensional electrophoresis and MALDI-TOF-TOF specific protein markers and obesity for obesity early protection factor. Its features need further validation.
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