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Objective quit smoking rat lung tissue nuclear factor-κB (NF-κB) and E-selectin expression levels of smoking and smoking cessation in patients with chronic obstructive pulmonary disease (COPD), lung tissue inflammation. Method 8-week-old male Wistar rats (Experimental Animal Center of Shanxi Medical) 32, (200 20) g body weight, were randomly divided into a control group, a small amount of smoking group, heavy smoking groups and smoking cessation group, n = 8 only. Refer to Xu Sanlin homemade experimental rat passive smoking device (automatic combustion device connected to a sealed glass box). Small amount of smoking group daily smoking, smoking 5 each, about 1 hour, smoking six days a week, 20 weeks a total smoking; heavy smoking group smoking 2 times a day, 10 per-smoking, about 2 hours smoking six days a week, a total of 20 weeks of smoking; smoking cessation group daily smoking and heavy smoking group, a total smoking after 20 weeks, 10 weeks out of the smoking environment; normal control group feeding, not smoking. Histological changes and expression by immunohistochemical method and in situ hybridization assay rat airway epithelial cell NF-κB and vascular endothelial cell E-selectin mRNA and protein levels. The one lung histology observed in control group rat alveolar structure normal continuous alveolar wall integrity, no inflammatory cells and glands of the small bronchi infiltration. Small amount of smoking group, heavy smoking groups and smoking cessation group rat alveolar walls with varying degrees of thinning or fracture, the alveolar structural disorder, respiratory bronchioles exist cystic dilatation, alveolar merging into bullae wall and surrounded by a large number of inflammatory cell infiltration, the ciliated epithelial broken off, cilia reduce heavy smoking group the most significant. Airway epithelial cells of NF-κB mRNA and its protein expression results with the control group, NF-κB mRNA and its protein level, a small amount of smoking group (0.41 ± 0.01,0.40 ± 0.01 (0.29 ± 0.03,0.28 ± 0.02) ), heavy smoking group (0.43 ± 0.01,0.41 ± 0.04) and smoking cessation group (0.39 ± 0.01,0.37 ± 0.03) expression levels were increased, and the differences were statistically significant (P lt; 0.05); heavy smoking group phase than a small amount of smoking groups and smoking cessation group NF-κB mRNA and protein expression levels decreased, and the differences were statistically significant (P lt; 0.05). Third, the vascular endothelial cell E-selectin mRNA and protein expression results with the control group of E-selectin mRNA and its protein (0.01 ± 0.04,0.02 ± 0.04) compared to the level of a small amount of smoking group (0.38 ± 0.02,0.43 ± 0.05), heavy smoking group (0.42 ± 0.01,0.52 ± 0.03) and smoking cessation group (0.36 ± 0.01,0.47 ± 0.05) expression levels were increased, the differences were statistically significant (P lt; 0.05); heavy smoking group compared to a small amount of smoking groups and smoking cessation group E-selectin mRNA expression and protein levels decreased, the differences were statistically significant (P lt; 0.05). , NF-κB and E-selectin mRNA and protein expression correlation using Pearson correlation analysis, the comprehensive analysis of NF-κB, E-selectin mRNA and protein expression of experimental data derived mRNA and protein expression of E-selectin NF-κB mRNA and protein expression was positively correlated, (r = 0.80, r = 0.89, P lt; 0.01). Conclusion Smoking can cause elevated rat airway epithelial cell NF-κB and vascular endothelial cell E-selectin mRNA and protein expression levels of both expression levels were positively correlated, and its expression levels increased with the increase of the amount of smoking, smoking cessation After the expression has declined, suggesting that quitting smoking can reduce airway inflammation, effective measures for prevention and treatment of COPD.
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