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Objective : To observe the PNS (PNS) on rat limb ischemia - reperfusion SOD, MDA and other indicators of impact and lower extremity skeletal muscle , blood vessels in the ischemic reperfusion TEM changes. Methods: ventral midline abdominal incision , the abdominal aorta artery occlusion clip rat lower extremity ischemia - reperfusion model of the sham group , ischemia group 1 hour , 2 hours ischemia group, ischemia 1 hour PNS treatment group, ischemia 2 hours PNS treatment groups. Determination of the rats plasma SOD, MDA content and transmission electron microscope skeletal muscle, vascular wall cells ultrastructural changes . Results: Compared with the sham group , ischemia- reperfusion group 1 hour increased MDA , SOD reduced obvious (P gt; 0.05), skeletal muscle cells less obvious changes in electron microscopy , a week after returning to normal. PNS treatment of ischemia 1 hour after 2 hours of reperfusion few filaments slightly blurred, mitochondrial swelling disappeared, stromal hyperplasia , ultrastructure than ischemia 1 hour 2 hours of reperfusion significantly improved . Ischemia and 2 hours of reperfusion was significantly higher when the MDA , SOD was significantly lower (P lt; 0.05). Ischemia and 2 hours after 2 hours of reperfusion limb skeletal electron microscope changed partially broken filaments , mitochondria reduced mitochondria become swollen sarcoplasmic increased. Ischemia and 2 hours PNS treatment group , the PNS (PNS) treatment, after 2 h of reperfusion clear sarcomere , mitochondria become swollen , spinal fracture. Ischemia -reperfusion 2 hours 2 hours after lower extremity vascular wall endothelial cells shrinkage, chromatin condensation , cytoplasmic you can see a lot of vesicle , wall large areas of necrosis , blocking the lumen , is irreversible change . Ischemia and 2 hours PNS treatment group , the PNS (PNS) treatment, after 2 h of reperfusion endothelial cell nucleus chromatin margination , intraluminal erythrocytes and granulocytes visible on both sides of the base film thickness varies Conclusion: rat abdominal aorta after 1 hour limb injury was mild , restore , blocking more than one hour after the various indicators changed much , severe skeletal muscle injury that may affect limb function , but after PNS ( PNS) treatment group after reperfusion injury lighter than the non- treated group , indicating that PNS (PNS) on ischemia - reperfusion showed a protective effect of abdominal aortic cross-clamping time should not exceed one hour.
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