Dissertation > Excellent graduate degree dissertation topics show

Experimental study on vascular endothelial function based on material changes in medicine winter \

Author: ZhangHua
Tutor: GuoXiaZhen
School: Beijing University of Traditional Chinese Medicine
Course: Basic Theory of Traditional Chinese Medicine
Keywords: Heart correspondence to summer vascular endothelial function substances Melatonin Yinyang of five Zang-organs and four seasons
CLC: R228
Type: Master's thesis
Year: 2010
Downloads: 85
Quote: 4
Read: Download Dissertation

Abstract

Theory of "Yinyang of five Zang-organs and four seasons " express that ma n is an integrated whole, and man and nature is an integrated whole. Theory of "Heart correspondence to summer "is the important content of theory of "Yinyang of five Zang-organs and four seasons ". The researches on theory of "Heart correspondence to summer " mainly focus on how heart adapt the changes of nature to mainta in the balance of physiological function.In recent years, the research of seasonal rhythm of cardiovascular disease has attracted attention of scholars. Studying on the seasonal climate influencing on physiological function is of great importance to clinical diagnosis, theory and preventing diseases.1. ObjectiveTaking the idea of "Heart correspondence to summer" in TCM as a breaking point,we research on the seasonal changes of vascular endothelial function materials to discuss the truth of theory of "Yinyang of five Zang-organs and four seasons ", which can enrich the scientific methods of theory of "Yinyang of five Zang-organs and four seasons”.Through this research,we can give some theoretic and experimental proofs on clinical diagnosis, theory and preventing diseases.2. Methods2.1 Theoretical methodsThe methods of the documentary substances and logical analysis were used to explain and analyze the physiological mechanism of "Heart correspondence to summer”.It showed that "Zang Fu proper to nature " had theoretical basis, and enriched the theoretical connotation of "Yinyang of five Zang-organs and four seasons ".2.2Experimental methods2.2.1 Experimental animalsTwenty-four SD male rats, weighted 120-180g,brought from Beijing WeitongLihua animal study center forty-five days before winter solstice and summer solstice were randomly divided into three groups:normal group, operation group(pinea lectomy group) and fake-operation group. All rats received na tural light, ate and drank freely, and were controlled under certa in room tempera ture was 17℃±2℃in winter, and it was 25℃±2℃in summer. Operation group and fake-operation group are operated a month before winter solstice and summer solstice.All the rats would be raised till winter solstice and summer solstice, on these days at 8 o’clock at night, they would be beheaded.2.2.2 Test methodsTo assay the ET-1 in plasma, Radio-immunity was engaged. To assay the contents of NO in serum, Nitrate reductase was engaged. To assay the contents of vWF in plasma, ELISA was engaged.2.2.3 Analysis method:All the data were showed by mean±standard error. The differences of groups were tested by SAS8.2. The values were calculated with Dunca n’s multiple range test or Student’s test. P< 0.05 meant obvious difference.3Results3.1 Results of theoretical study After analysis of the literature of the theory of "Heart correspondence to summer ",we think the meaning of this theory is:summer is the season which easy to get cardiovascular disease, winter is the season which cardiovascular disease easy to deteriorate.3.2 Results of experiments3.2.1 Seasonal change can influence In normal stasis, the vascular endothelial function substances have the character of seasonal changes.(1) ET-1 in plasma has significant difference between winter and summer (P＜0.0001). It was higher in winter and lower in summer.(2)NO in serum has significant difference between winter and summer (P＜0.0001). It was higher in summer and lower in winter.(3)vWF in plasma has significant difference between winter and summer (P＜0.0001). It was higher in winter and lower in summer.These results indicate that winter and summer changs has significant impact on vascular endothelial function substances. ET-1、vWF is higher in winter than in summer, NO is higher in summer than in winter.3.2.2 Pineal gland-Melatonin can influence on the seasonal changes of vascular endothelial function substances.If we remove the Pineal gland, the seasonal changes of vascular endothelial function substances can be destroyed:(1) In winter,normal group of ET-1 in plasma was significant higher than operation group of it (P＜0.01). There is no difference between normal group and fake-operation group. After removed the Pineal gland, There is no difference of ET-1 in plasma between winter and summer. Fake-operation group of ET-1 in plasma was significant higher in winter than in summer (P<0.01). In summer,normal group of ET-1 in plasma was significant lower than operation group of it (P＜0.01). There is no difference between normal group and fake-operation group. After removed the Pineal gland, There is no difference of ET-1 in plasma between winter and summer. Fake-operation group of ET-1 in plasma was significant higher in winter than in summer (P＜0.01)(2)In winter,normal group of NO in serum was significant lower than operation group of it (P＜0.01). There is no difference between normal group and fake-operation group. After removed the Pineal gland, There is no difference of NO in serum between winter and summer. Fake-operation group of NO in serum was significant higher in summer than in winter (P＜0.0001). In summer,normal group of NO in serum was significant higher than operation group of it (P＜0.01). There is no difference between normal group and fake-operation group. After removed the Pineal gland, There is no difference of NO in serum between winter and summer. Fake-operation group of NO in serum was significant higher in summer than in winter (P＜0.0001)(3)In winter,normal group of vWF in plasma was significant higher than operation group of it(P＜0.0001). There is no difference between normal group and fake-operation group. After removed the Pineal gland, There is no difference of vWF in plasma between winter and summer. Fake-operation group of vWF in plasma was significant higher in winter than in summer (P<0.0001).In summer,normal group of vWF in plasma was significant lower than operation group of it (P＜0.0001). There is no difference between normal group and fake-operation group. After remove the Pineal gland, there is no difference of vWF in plasma between winter and summer. Fake-operation group of vWF in plasma was significant higher in winter than in summer (P<0.0001)These results indicate that Pineal gland-Melatonin can influence on the seasonal changes of vascular endothelial function substances. If we remove the Pineal gland, there is no difference of these substances between winter and summer.3.2.3seasonal changes of ET-1/NOIn normal stasis, ET-1/NO has significant difference between winter and summer (P＜0.0001). It was higher in winter and lower in summer. The means are 3.983±0.611 and 1.044±0.406 respectively.After removed the Pineal gland, There is no difference of ET-1/NO between winter and summer(P<0.0001). The means are 2.056±0.237 and 2.044±0.438 respectively. The results of fake-operation group are similar as normal group.These results indicate that seasonal changes can imbalance the contents of ET-1 and NO. In winter ET-1 is higher than NO and in summer ET-1 and NO are almost equal. What’s more, these changes are influenced to the Melatonin. If we remove the Pineal gland, these changes are not obviously.3.2.4Correlation between vWF and ET-1、NOIn normal stasis, it has positively correlation between vWF and ET-l,(P<0.01) and negative correlation between vWF and NO(P<0.01). The results of winter and summer are similar.If we remove the Pineal gland, these correlations not disappear.The results of fake-operation group are similar as normal group.These results indicate that it has positively correlation between vWF and ET-1 and negative correlation between vWF and NO. These correlations are not the result of melatonin.4 Conclusion4.1 In normal stasis, the vascular endothelial function substances have the character of seasonal changes.4.2 The seasonal changes of vascular endothelial function substances are influence to Pineal gland.4.3 Seasonal changes can imbalance the contents of ET-1 and NO. In winter ET-1 is higher than NO and in summer ET-1 and NO are almost equal.4.4 It has positively correlation between vWF and ET-1 and negative correlation between vWF and NO.

Related Dissertations

CLC: > Medicine, health > Chinese Medicine > Basic Theory of Traditional Chinese Medicine > Chinese medicine pathological
© 2012 www.DissertationTopic.Net Mobile