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The Role of AFP in Glucocorticoid Induced Premature in Rat Colon of Development

Author: LiuXiaoYan
Tutor: GeYingBin
School: Nanjing Medical University
Course: Physiology
Keywords: Alpha-fetoprotein Hydrocortisone Development Mesenchymal Colon Rats
CLC: Q57
Type: Master's thesis
Year: 2009
Downloads: 18
Quote: 0
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Abstract


Glucocorticoids is considered to be the important regulatory factors for multiple organ system maturation process, given within the first two weeks of postnatal exogenous glucocorticoids can cause gastrointestinal precocious, but the exact mechanism is still unclear. Studies have shown that the role of promoting premature not the direct role of the glucocorticoid effect, but glucocorticoid and other tissues, cells, especially mesenchymal cells together results. Alpha-fetoprotein (alpha-fetoprotein, AFP) is an important growth factor during the development of the gastrointestinal tract, has been reported, within the digestive tract of the AFP is mainly present in the mesenchymal cells. Therefore, we speculate, may be involved in promoting precocious role of glucocorticoids in the digestive tract of the AFP. We will experiment was divided into two parts: 1. Clear AFP expression and localization in different developmental stages of rat colon; study the expression of AFP with exogenous glucocorticoid-induced colon advance development process was in contact with. First part: the rat embryo, 18.5 days (embryonic day 18.5 e18.5) to e20.5, born the same day (postnatal day 0, p0), p7, p14, p21, and adult (8w) colon tissue by RT -PCR and immunoblotting to detect the expression levels of AFP, Immunofluorescence double staining identified AFP cytology positioning. Found that the Western blot detected only the 72kDa subtypes AFP expression; expression of AFP mRNA and protein levels in rat embryos highest 18.5 days, then decreased gradually, not detected in adult rats; detection period , AFP and vimentin (vimentin) are co-expressed in mesenchymal cells. Second part: We selected rats born after the first 7 days, 9 days, 11 days, subcutaneous injection of drugs, the following experimental groups: 1) the volume of the saline control group; 2) 100μg / g of hydrocortisone ( hydrocortisone, HC) treatment group; 3) 100μg / g HC and 100μg / g mifepristone (the specific glucocorticoid cytoplasm receptor antagonist) to treatment groups; 4) 100μg / g of mifepristone treatment group . Then in the first eight days after birth, 10 or 14 days to extract colon tissue. Using hematoxylin - eosin staining observed morphological changes of the colon, muscle tension assay detects colon dynamics; expression of AFP detected by RT-PCR and immunoblotting techniques; Immunofluorescence double staining technique used to identify AFP cytology positioning. The results found that exogenous HC can promote the lactating rat colon morphology and function of early development, and accompanied by upregulation of AFP mRNA and protein and AFP-positive cells increased. Mifepristone significant increase in protein expression reversed the HC the induction of AFP. The above results show that the colon of the AFP is produced by the mesenchymal cells; lactating exogenous glucocorticoids can promote early development of the morphology and function of the rat colon, in this process, the amount of AFP Expression of rising; exogenous glucocorticoid promote the early development of rat colon may be raised achieved through the expression of AFP.

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CLC: > Biological Sciences > Biochemistry > Hormone
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