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The papers discussed the developed status quo and development on the basis of the recent years and abroad microbial flocculant to generate screening of bacteria MF-04, alternative medium, optimization of culture conditions, the flocculating characteristics, flocculation mechanism of microbial flocculant applications, as well as the research and discussion, the main content and conclusions are as follows: (1) In this experiment, paper mills on laboratory and Pumiao of Environmental Engineering, College of Chemistry and Chemical Engineering, Guangxi University activated sludge bacteria source, rich in a particular medium after set training, separation and screened 14 strains with high flocculating activity, including one pair of Kaolin high flocculating activity, 87.1% named MF-04. In order to save costs, find a cheap material and sugar cane juice as an alternative medium. Effects of applied nitrogen, phosphorus source flocculating activity. Also conducted research on the synthetic medium. (2) In order to improve the flocculating activity, based on the fermentation medium in the generic, carbon, nitrogen, K 2 HPO 4 concentration, the medium pH, Shaker speed condition optimize. And various factors in the order of the size of the flocculating activity and optimal conditions by orthogonal experiment. (3) MF-04 produced flocculants for kaolin suspension pH value, Jing Shen time, flocculant dosage of CaCl 2, dosage amount, stirring speed, high-temperature processing, flocculant The conditions for the conditions of the storage time verification, obtained under various conditions, the optimum value. Flocculant - producing obtained MF-04 The growth curves were obtained MF-04, the optimal growth period 60H. (4) bacteria MF-04 produced flocculant extraction and purification, to find that the main component of the flocculant is a polysaccharide, and the polysaccharide content measured by ultraviolet and infrared spectra. Found after in-depth study by Zeta potential and infrared spectra of the flocculation mechanism, Ca 2 monodentate bridging flocculation mechanism for adsorption bridging mechanism,-COO - key and mechanism of mode. (5) by orthogonal and single factor was found by bacteria MF-04 producing flocculant has a good effect of mineral processing waste water turbidity and COD removal. And PSAZ mixed, turbidity is not only lower than when used alone, and also to reduce the total dosage of flocculant. Comparison, the treatment effect is superior to the inorganic polymer flocculant and inorganic polymer flocculant; better treatment effect, brown sugar decolorization bacteria MF-04 producing flocculant and polydimethyl diallyl ammonium chloride compound, brown sugar decolorization rate of up to 38.9%.
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