Dissertation > Excellent graduate degree dissertation topics show

Isolation and Identification and Parts of Biological Characteristics of Pasteurella Multocida Inducing Pneumonia of Calf

Author: MaXiaoZuo
Tutor: ZuoGenQiang
School: Shihezi University
Course: Preventive Veterinary Medicine
Keywords: Calf pneumonia Pasteurella multocida A type of capsular serum Isolation and identification of PCR Molecular biology
CLC: S852.61
Type: Master's thesis
Year: 2010
Downloads: 108
Quote: 0
Read: Download Dissertation


Kill more Pasteurella the (Pasteurella mutocida, Pm) is the main pathogens of the bovine hemorrhagic septicemia, sporadic or endemic diseases caused by bacteria, mainly as sepsis and hemorrhagic inflammation, fever, pneumonia or acute gastric enteritis and visceral extensive bleeding as the main feature. At home and abroad caused by bacteria calf pneumonia has rarely been reported. In 2007, Shihezi, Xinjiang has six large-scale dairy farms 1-9 weeks old calves sudden onset, a total of more than 90 calves died, the main clinical symptoms of fever, cough, diarrhea is characterized by. In order to determine the cause the calf disease cause of death, to avoid the spread of the disease and effectively control the spread of the disease. Acquisition incidence calves diseased Pathogen culture characterization, physiological, biochemical and molecular biological identification, animal infection test isolates, detection of virulence factors and susceptibility testing. The results are as follows: 1,4 isolates were gram-negative short bacillus, rounded at both ends. Isolates morphological characteristics and cultural characteristics consistent with previous cause of adult cattle sepsis like Pasteurella isolates biochemical identification results and kill more the Pasteurella biochemical characteristics. Reference kill Pasteurella species specific gene kmtl and the capsular serotype specific gene HYAD-HYAC bcbD dcbF ecbJ, fcbD sequence to design primers for four isolates with multiplex PCR amplification results isolates could be amplified less than 500bp and 1000bp bands, Actinobacillus pleuropneumonia, enterococci and E. coli as a template can be amplified specific bands, send the sequence in GenBank nucleotide than , indicating that the isolates were capsular serum type A Pasteurella multocida. And those who primer Kmtl amplification for positive strains biochemical results are in line with the PM standard. Pure culture isolates were inoculated mice, rabbits, experimental animal pathogenic isolates showed strong pathogenicity test group of mice all died within 48h, the rabbits were 72 hours after death, can be recovered from the dead rabbits and mice inoculated with bacteria. 4, susceptibility test results show that the separation of 4 pm ofloxacin, gentamicin higher sensitivity. To ciprofloxacin, neomycin sensitivity is not high, but for ampicillin sodium sensitivity. In our study, more than kill Pasteurella multocida the iron uptake related tbpA gene encoding type 4 of pili ptfA gene, isolates the detection of virulence factors, the results of sequencing analysis detected only ptfA genes. From calves, mice, rabbits pathological histological changes visible primary lesions in the lungs, alveolar wall can be seen telangiectasia congestion, alveolar serous edema, alveolar cellulosic exudate, which shows a small amount of neutrophils and erythrocytes. Other organs are varying degrees of bleeding. The results confirmed that the isolates of Pasteurella multocida. This is the first confirmed Xinjiang region killed Pasteurella multocida capsular serum caused by type A calf pneumonia, and systematic study of the Xinjiang Pasteurella multocida biological characteristics, while establishing a common biochemical identification to molecular biology fast detection diagnostic mode.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Studies on the Quality of Phisio-Chemical and Fresh Keeping Technologies of Post-Harvest in Volvariella Volvacea,S646.13
  3. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  4. Directed Evolution of Lipase from Penicillium Expansum FS1884 by Error Prone PCR,Q78
  5. Mutagenesis of a Laccase Gene from Pleurotus eryngii in Vitro and Expression in Pichia Pastoris,TQ925
  6. Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
  7. Identification of Cyto-and Molecular Genetics of Multisomic Addition Line "Nj08-063" in Brassica Napus and Its Agronomical Traits,S565.4
  8. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  9. The Detection of the Virulence-related Gene from Vibrio Alginolyticus and the Study on Preservation Methods,S943
  10. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  11. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4
  12. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  13. Control of Verticillium Wilt Disease of Cotton Plants with the Application of a Bio-Organic Fertilizer and Its Microbiologecal Mechanism in Rhizosphere,S144.1
  14. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  15. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  16. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  17. Analysing Sequence Characters of ADSL and PurH Gene and Correlation between Genes Expression and IMP Content in Duck,S834
  18. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  19. Isolation and Identification of Avian Pathogenic Escherichia Coli from Chicken and Characterizations of Its Virulence-Associated Genes,S852.61
  20. Isolation and Identification of Infectious Bronchitis Virus and Sequence Analysis of Its S1 Gene and N Gene,S852.65
  21. Isolationand Identification of Porcine Parvovirus and Parts of Its Biological Characteristics,S852.65

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Pathogenic bacteria
© 2012 www.DissertationTopic.Net  Mobile