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Select Breeding and Structure Optimization of Syntrppuic Acetogens Coculture B6

Author: GaoChenChen
Tutor: LiJianZheng
School: Harbin Institute of Technology
Course: Microbiology
Keywords: Anaerobic digestion Production of hydrogen acetogen The mutual Camp cultures Breeding Colony composition Optimization
CLC: X172
Type: Master's thesis
Year: 2010
Downloads: 102
Quote: 1
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Abstract


In anaerobic digestion, is located on the trophic niche the acidogenic fermentation flora and methanogenic flora between the hydrogen-producing acetogenic flora, due to its interaction with the hydrogen-consuming bacteria camp symbiotic characteristics its isolation and culture is very difficult very scarce resources, seeds, limits based on strengthening the development of the production of hydrogen production acid function of the high-rate anaerobic biological treatment technology. In comparison, the hydrogen production acetogen camp each co-culture Isolation and screening is relatively simple, seed resources more accessible, is expected to be based on the development of a breakthrough to enhance production of hydrogen production acid function of the high-rate anaerobic biological treatment technology. Papers on the pre 7 hydrogen production acetogen camp each co-culture body rejuvenation basis, through culture experiments proved the law of population growth, and to further define the best time of inoculation training for groups of 48 9 6 h; study and comparison of physiological and biochemical experiments and hydrogen production producing acetic acid metabolism intensity from preferably a propionic acid as a substrate proliferation and production of hydrogen produced the strongest acid metabolism inter-camp of cultures B 6 . Camp each from the optimization of production of hydrogen acetogen co-cultured cultured point of view, research and to design the hydrogen production acetogen the basal medium, and effects of temperature, pH, exhaust, inoculation ratio of co-cultures of B 6 growth and metabolism and the production of hydrogen acetic acid production capacity. The results showed that in 42.5 ° C, pH 7.2, at atmospheric pressure exhaust gas, the volume of the inoculum suspension with the liquid medium under conditions with a ratio of 4:10, of culture body B 6 can exhibit the most good substrate conversion, proliferation, and production of hydrogen acetic acid production capacity. To strengthen hydrogen production acetogenic mutual camp co-culture the body B 6 of hydrogen-producing acetic acid production role, determined by studying B 6 on excellent medium, subculture and culture conditions, and flora is more simple, more prominent hydrogen-producing acetogenic metabolism characteristics inter-camp training the body B 6 -8c. PCR-DGGE technical analysis, mutual camp co-culture only four of the presence of bacteria in the body B 6 -8c less than B 6 3. Flora composition analysis, the B 6 -8c and B 6 containing obligate interactions camp production the hydrogen acetogen Peptococcus-sp and its associated bacteria associated bacteria, iron-reducing bacteria, Clostridium sp in the metabolic process does not produce CH 4 or H 2 S, but the remaining hydrogen and acetic acid. B 6 -8c exclusion of the original B 6 such as the production of hydrogen acetogen contains symbiotic bacteria: the use of acetic acid and hydrogen Sedimentibacter sp and degradation of amino acids, benzoic acid, toluene, and the like bacteria Clostridium hydroxybenzoicum.

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