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Preparation and Characterization of MWCNTs and Its Application of Electrochemical Biosensor

Author: ZhengYanJie
Tutor: LinXinHua
School: Fujian Medical
Course: Pharmaceutical Analysis
Keywords: Multi-walled carbon nanotubes Characterization DNA electrochemical biosensor CML gene
CLC: TB383.1
Type: Master's thesis
Year: 2008
Downloads: 336
Quote: 0
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Abstract


DNA electrochemical biosensor was developed rapidly in recent years, a new bio-sensor with a high degree of specificity of DNA hybridization reactions, but also has high sensitivity electrochemical sensor, fast response, simple operation, miniaturization, low prices, and other significant advantages at home and abroad in recent years become a research hotspot. DNA electrochemical biosensor is one of the key technologies developed high-sensitivity screening electrode material. In this thesis, carbon nanotubes as a raw material for the first time using ultrasound - microwave assisted extraction was prepared by acidification of multi-walled carbon nanotubes, developed the use of carbon nanotubes modified DNA electrochemical sensor for the solution of the quantitative detection of complementary DNA and Based on this indicator explores the role of time, the role of hybridization buffer solution concentration and the impact on the electrochemical detection; also investigated the stability of the sensor, specificity and other basic properties. Using scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), scanning probe microscopy (SPM), electron diffraction spectroscopy (EDS), infrared spectroscopy (FTIR) methods such as carbon nanotubes obtained to characterize the results show that in a 3:1 mixed acid (concentrated sulfuric acid: concentrated nitric acid) 40oC ultrasound - Microwave Synergistic Extraction of 40 minutes, get high quality and high purity carbon tube function, and the results easily repeatable, easy to operate. Multi-walled carbon nanotubes on the catalytic performance of Preliminary found that the acidification of carbon nanotube modified electrode applied to small biomolecules acetaminophen (ACOP) detection can significantly improve the ACOP electrode reaction reversibility, high sensitivity, response fast and good selectivity. The method for producing a DNA electrochemical biosensor for chronic myeloid leukemia disease gene sequence analysis showed that: in PBS (pH7.0) buffer solution, indicator daunomycin concentration 10μM, hybridized with fully complementary single-stranded 45oC hybrid 40 minutes, the detection signal to good effect. Using DNA electrochemical sensor detects standard DNA samples, the detection limit reached 8.0 × 10 -10 mol / L, at 4.0 × 10 - 9 ~ 1.0 × 10 - 7 mol / L concentration range, the detection signal with a linear relationship between the concentration of the complementary DNA, and have a good reproducibility and specificity. Compared with the bare electrode, MWNTs can significantly improve the DNA-modified electrode in a fixed amount, and the intercalator can accelerate the rate of electron transfer between the electrodes, thus improving their complementary DNA sequences on the detection response speed and sensitivity.

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