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Study on Screening of High Efficient Strains for Shenfu Coal Bioconversion

Author: WangYongJuan
Tutor: ZhouAnNing
School: Xi'an University of Science and Technology
Course: Applied Chemistry
Keywords: Shenfu coal Biotransformation Strains preferred Light / bio-oxidation coupling
CLC: TQ530.2
Type: Master's thesis
Year: 2008
Downloads: 129
Quote: 4
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Abstract


Shenfu coal, the paper as the research object, extract from Shenfu coal washing wastewater strains Shenfu coal weathering and photo-oxidation of coal as the sole carbon source Filter by strains preferred in order to obtain efficient coal solubilization strain; Research and light oxidation God House of coal biotransformation characteristics of the biotransformation structure of the product, to provide experimental evidence to clarify the relationship between the coupling of photo-oxidation of coal biotransformation. First, the coal samples UV catalytic oxidation pretreatment, photo-oxidation time 6h, humic acid content reaches a maximum of about 10.19%. Elemental analysis and FTIR analysis showed that the light oxidation pretreatment of coal in the O / C atomic ratio and H / C atomic ratio increases, the content of the ether bond, a carboxyl group and a phenolic hydroxyl groups increase. 3 differential medium (identification of fungi, actinomycetes and bacteria) from Shenfu coal washing waste water separation and purification of the 47 strains. Shenfu of weathered coal as sole carbon source by the inorganic salt agar plate culture screening, photo-oxidation of coal the sole carbon liquid dynamic culture rescreening, preferably three kinds of strains, namely b bacteria (fungi), g bacteria (actinomycetes) and h bacteria (bacteria). Culture methods on microbial transformation, the results show that: the inorganic salt culture strains dissolved coal is better than organic medium strains; dynamic method compared with static culture, the strains of the former coal solubilization better. Dynamic inorganic culture conditions, the best solubilization cycle is 14 days, and the acidic substance generated in the coal biotransformation process. Photo-oxidation pretreatment of coal samples with high biological conversion rate, in which g bacterium light oxidation pretreatment of the coal sample conversion rate of up to 22.3%. Coal by b bacteria, g bacteria and h bacteria into liquid pale yellow, raw coal b bacteria transformation products, all the liquid transformation products after alkali treatment, have a brown-yellow precipitate, photo-oxidation of coal g add alkaline precipitation of the bacteria into the liquid product formation rate up to 17.5%. FTIR analysis showed that the precipitated product containing a hydroxyl group, an ether bond, an aromatic ring, such as a functional group. Biotransformation of residual coal O / C ratio, H / C ratio is significantly increased. g bacteria transformed residual coal ether bond functional group content decreased, indicating g bacteria is readily transformed into the ether bond-containing substances. The above results show that the light oxidation treatment promotes coal biotransformation. Cascade solvent extraction method in a Soxhlet extractor with acetone and tetrahydrofuran (THF) as solvents, photo-oxidation and biotransformation of residual coal composition. The results showed that the photo-oxidation of the coal acetone extraction rate decline, but its acetone extraction of residual coal the THF extraction rate more than 2 times; after biotransformation, the residual coal acetone extraction rate is up sharply acetone extraction the THF extraction rate of the residual coal has increased strain type. Raw coal g bacteria into acetone extraction rate increased to the maximum extent, up to 14.96%, after conversion of the photo-oxidation of coal by g bacteria, acetone extraction rate of increase raw coal; compared to raw coal, the photo-oxidation of coal by b bacteria and g bacteria into the acetone extraction the THF extraction rate of the residual coal increased nearly doubled, acetone extraction rate of photo-oxidation of coal by b bacteria into the greatest increases, and acetone and THF extraction yields the highest sum 21.47%, 2.92% the original pulverized coal extraction rate, the addition the photooxidation of coal h bacteria organisms transforming. Therefore, the the Shenfu coal in bacteria, b and g bacteria biological processes and photo-oxidation pretreatment with a coupling effect. FTIR analysis showed that after biotransformation, the residual coal acetone extract the relative reduction of carbonyl and ether bond functional groups.

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CLC: > Industrial Technology > Chemical Industry > Processing and utilization of coal chemical and coal > Basic theory of the coal chemical > Coal pyrolysis and conversion
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