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Pleurotus ostreatus (Pleurotus ostreatus), commonly known as oyster mushroom, the English called Oyster Mushroom, classified as belonging to the Basidiomycota (Basidiomycota), the layer Hymenomycetes (Hymenomycetes) the Agaricales (Agaricales), pleurotaceae (Pleurotaceae) PLEUROTUS (Pleurotus). In this study, mRNA differential display analysis of growth of oyster mushroom mycelium and fruiting bodies in the development process of differentially expressed genes. Ostreatus 739 the four stages of growth and development of mycelium, primordium, Gulei and mature fruiting bodies of total RNA as a template, with the T 12 M (M for G, A, C ) three kinds of anchor primers matter and 14 kinds of random primer combination DDRT-PCR by 8% denaturing PAGE gel electrophoresis and silver nitrate staining, a total get 130 difference bar with about 300 to 1500 bp fragment length, on which 17 a differentially expressed cDNA fragments were cloned and sequenced, and sequencing results by GenBank BLAST search. The results showed that Seq1, SEQ2, and the SEQ3 with known genes have high homology, while the other fragment with low homology to known genes. SEQ1 for 421bp ostreatus an unknown gene, which is expressed in the mycelium was not significant, but more strongly expressed in the primordia, Gulei and fruiting bodies, similar to those found in the gray Coprinus and mushrooms . Seq2 607bp, its expression in the mycelium significantly stronger than the primordia, Gulei and mature fruiting bodies, high homology with malate dehydrogenase (MDH), MDH the glycolytic pathway and a very important enzyme in the citric acid cycle, which may be involved in the mycelium develops into the primordia process. Some active physiological and biochemical activities, such as energy metabolism. SEQ3 293bp, Coprinus gray an unknown protein has a high homology, at the same time, it is with the rod Aspergillus peptidyl-prolyl isomerase (PPIase) has some homology, and PPIase negative regulation of plant MYB transcription factor. MYB factor through the optical signal of plants for the regulation of physiological regulation and development patterns, so we guess SEQ3 ostreatus differentiation and development to a certain extent. A low homology fragments may be unknown gene, these fragments further research will help to get the complete gene and understanding of Pleurotus ostreatus mycelium and fruiting bodies of the mechanism of differentiation and development.
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