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Enrichment and Distribution of Germanium in Ganoderma Lucidum

Author: WangHongGeng
Tutor: GuoZiJian
School: Agricultural University of Hebei
Course: Plant Physiology
Keywords: Germanium Ganoderma Digestion Graphite furnace atomic absorption spectrometry Enrichment Distributed Mycelium Fruiting bodies
CLC: S567.31
Type: Master's thesis
Year: 2001
Downloads: 290
Quote: 9
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Abstract


Ganoderma (Ganoderma lucidum) is a precious treasure house of Chinese medicine in the medicinal and edible fungus. Is generally believed that, in addition to the Ganoderma lucidum polysaccharides, amino acids, Ganoderma lucidum acid, sterol efficacy of Ganoderma its relatively abundant organic germanium related. This study to establish a simple, fast, accurate and reliable determination of trace germanium; and explore the enrichment patterns of germanium in Ganoderma. The Determination of Trace Germanium in Ganoderma samples by graphite furnace atomic absorption spectrometry. The results show that: HNO 3 -H 2 O 2 the ideal digestive juices; the digestion temperature should be controlled at about 120 ℃; samples digestion 12 hours to be immersed in the digestive juice; total digestion time of not less than 26 hours; plus H 2 O 2 digest completely, but also to remove the residual the HNO of 3 . The digestion method recovery was 95.7% to 98.7%. Using Pd (NO 3 ) 2 and Sr (NO , 3 ) 2 mixed matrix modifier, avoid germanium volatilization losses, improve the ashing temperature to 1400 ° C to eliminate the interference of the matrix. The instrument method recovery was 95.1% to 100.3%, and the detection limit was 0.154μg / L, and the relative standard deviation of 3.8%. This paper also discusses the germanium Ganoderma growth and Ganoderma lucidum germanium enrichment law, in Ganoderma medium by adding different concentrations of inorganic germanium, germanium content of the different parts and different periods of growth and fruiting bodies of Ganoderma the analysis found from medium - mycelium - primordium - fruiting bodies (stipe - cap - Junzhe) - spore powder, the absorption of germanium delivery preaching. Ganoderma growth of various periods and various parts of the fruiting bodies grow in different germanium concentration gradient medium, the enrichment of germanium meet the logistic equation, the increase in the amount of germanium slow - fast - slow process. The mycelia in the medium germanium concentration is higher than about 100mg / L severe antimicrobial effect, and with the increasing concentration of heavier; fruitbody 200mg/Kg serious inhibitory effect is higher than in the medium germanium concentration. The culture rich germanium ganoderma medium germanium content of mycelium 100mg / L, and the fruiting body 100mg/Kg ~ 200mg/Kg wet weight is appropriate. Ganoderma different periods of the order of the amount of germanium: mycelium spores on fruiting body> primordia. Ganoderma in different parts of the order of the amount of germanium: stipe> gill> cap. Ganoderma lucidum primordium of the different periods of development of germanium content minimum; cap in different parts of the fruiting body containing germanium lowest. Ganoderma growth and development characteristics. The primordia period is in the cell division exuberant period; gill differentiation comes from the cap on, this process of cell division is also a strong period of lower germanium content primordia differentiation and gill primordium and pileus the formation has important significance.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Fungus > Ganoderma lucidum
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