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Detection of Horseradish Peroxidase with Resonance Light Scattering and Functionalized Carbon Nanotube and It’s Applications in Bioassay

Author: GaoRuiGuang
Tutor: LiZhengPing
School: Hebei University
Course: Applied Chemistry
Keywords: Horseradish peroxidase Carbon nanotubes Resonance Light Scattering Fluorescence
CLC: Q946
Type: Master's thesis
Year: 2009
Downloads: 27
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Abstract

The enzyme is produced by cells having a catalytic function of the protein, enzyme analysis and has an important meaning in life science research , and has a profound impact on the biological, chemical , and other fields . But conventional enzyme detection methods , such as a radioactive marker, an enzyme-labeled method, etc. , often complicated operations , restriction enzyme analysis of the studies on this to a certain extent . With the development of the life sciences , and how the enzyme is fast, simple and efficient detection has become a hot research topic . Horseradish peroxidase is the most widely used of an enzyme preparation . Carbon nanotubes , discovered in the 1990s , a new type of material , its unique physical and chemical properties , has become in many areas of research focus . The paper is divided into two parts , both widely used substance made ??research , Environmental Microbiology immunoassay for the development of a wider range of applications . The first part , based on the oxidation reaction of phenol with H2O2 resonance light scattering technique for determination of horseradish peroxidase . A high sensitivity of determination of the horseradish peroxidase resonance light scattering analysis . HRP-catalyzed phenol and H2O2 reaction phenolic conjugated polymers to cause resonance light scattering signal between 200 ~ 700 nm has been greatly enhanced , and reaches a maximum at 313 nm . Based on this phenomenon , the establishment of a sensitive method of detection of HRP . Determination The linear range of the standard curve of HRP is 0.05 ~ 5.0 × 10-7g/mL, the detection limit is 1.1 × 10-10 g / mL . The second part, the chemical modification of the carbon nanotubes and the DNA molecule functional. This article using a simple method of the carboxylated carbon nanotubes modified by covalent interaction with the single-stranded DNA binding , using the electrostatic binding of the conjugated polymers PFP with DNA having strong fluorescence , to achieve the function of DNA molecules of carbon nanotubes of validation , establish an easy method of carbon nanotubes functionalized carbon nanotubes as a function of fluorescent probes in immunoassay applications and lay the foundation for the development of a carbon nanotube immunoassay environmental Microbiology application

Detection of Horseradish Peroxidase with Resonance Light Scattering and Functionalized Carbon Nanotube and It’s Applications in Bioassay

Abstract

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