Dissertation > Excellent graduate degree dissertation topics show

Indirect Imaging of Singlet Oxygen Generation from a Single Cell

Author: ShenYi
Tutor: LiBuHong
School: Fujian Normal University
Course: Optical Engineering
Keywords: photodynamic therapy singlet oxygen fluorescence probe intracellular uptake subcellular localization SOSG diffusion reaction kinetic curves
CLC: Q2-3
Type: Master's thesis
Year: 2011
Downloads: 3
Quote: 0
Read: Download Dissertation

Abstract


Indirect detection technology with high sensitive and high selective fluorescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photophysical and photochemical behavior of Singlet Oxygen Sensor Green (SOSG), a commercially available water-soluble fluorescent probe for singlet oxygen, was examined at first. The obtained results indicate that the fluorescence probe has high sensitive and selective detection for 1O2. In addition, thought SOSG and SOSG-EP is the efficient singlet oxygen photosensitizer, SOSG can be used effectively to detect the production of 1O2 with proper irradiation time. Second, in this study we use SOSG to study the 1O2 diffusion in single intact living cells. Our findings indicate that SOSG holds great promise for the indirect imaging of the 1O2 that can escape from single intact living cells. Depending on the site of’02 generation, 1O2 generated in the plasma membrane treated with PpIX can escape from the cell interior into the extracellular environment, while the 1O2 generated in the nucleus cannot.

Related Dissertations

  1. Research on Methods of Medical Ultrasound Image Denoising,TP391.41
  2. cDNA Cloning, Expression of vp5 and vp7 Genes and Subcecullar Localization of VP5 and VP7 Proteins in Grass Carp Reovirus,S941.41
  3. Hot air drying characteristics of lettuce osmotic dehydration mass transfer kinetics and permeability,TS255.52
  4. Reaserch on the Effect of Fabric Structure on the Performance of Seamless Sports Underwear,TS941.15
  5. Expressive Characterization of Transcription Factors in Cotton Fibre Initiation and Cloning and Characterization of Three Transcription Factor Genes in Gossypium Hirsutum L.,Q943
  6. Analysis of Concrete Durability under Combined Action of Freeze-thaw Cycles and Chloride Ion Erosion,TU528
  7. Cloning and Characterization of Photoperiod Sensitive Gene ZmELF4 in Maize,S513
  8. The Clinic Value of Diffusion Weighted Whole Body Imaging with Background Body Signal Suppression (DWIBS) Compared with hPET/CT,R445.2
  9. Drug Diffusion in Optimizing Control and Its Numerical Method,R91
  10. Study on Reaction-diffusion Model of Educational Innovation,G40
  11. Expression Analysis and Localization of OsDMI3 Gene Induced by Abscisic Acid,S511
  12. Cloning and Expressing Profiles of Eimeria Tenella Rhoptry Neck Protein 2 EtRON2 Gene during the Different Development Stages,S852.7
  13. Diffusion Tensor Imaging Study of Idiopathic Epilepsy at 3.0T High Field Magnetic Resonance Scaner,R445.2
  14. The Value of Diagnosis for Prostate Cancer by Combined Use of MRS and DWI,R737.25
  15. The Research on Teacher Professional Knowledge Diffusion Path of Teachers Groups in the Information Technology Environment,G451.1
  16. Identification of Salt Tolerance and Function Analysis of NHX1 Gene in Glycine Max, Glycine Soja and Their Hybrid Seedlings,S565.1
  17. Research on Super-resolution Reconstruction in SAR Image,TN957.52
  18. First-Principles Studies of Blacl Phosphorus and LiMn2O4 as Electrode Material for Lithium Ion Batteries,TM912
  19. Determination of Organophosphorus Pesticides Residues by Fluorescence Method and Its Application,O657.39
  20. First-Principles Studies of Cathode Material Srn+1TinO3n+1（n=1,2,3,∞） for Solid Oxide Fuel Cell,TM911.4

CLC: > Biological Sciences > Cell Biology > Cell Biology Research Methods
© 2012 www.DissertationTopic.Net  Mobile