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This research is mainly about adding xylanase xylanase diet nutrient content and metabolism of broilers relationship to study diets xylanase preparations xylan content of dry matter on broiler energy, calcium, phosphorus and nitrogen metabolic rate, and the establishment of dietary xylan content of nutrients between metabolic rate mathematical model for a reasonable diet xylanase preparations provide a theoretical basis. Test 1 with D-xylose as a standard substance, orcinol - hydrochloride as chromogenic agent, established a dual wavelength absorbance measurement difference between xylan content of the feed raw material in method, when the reaction solution, the concentration of D-xylose 0 ~ 100μg/ml, its absorbance changes consistent with Lambert-Beer's law, the standard curve regression equation was y = 0.0039x-0.0151 (x for dual wavelength absorbance difference, r = 0.9972). The results show that this method is simple determination, high recovery, good repeatability, the relative error is small, can be used for feed ingredients xylan content; using this method measured 10 kinds of raw materials xylan contents were: wheat was 6.88%, rice bran was 15.06%, 4.54% corn, soybean meal was 5.81%, 6.70% barley, sorghum was 2.69%, wheat bran was 16.78%, the powder was 9.09%, to 12.64% cottonseed meal, rapeseed meal to 8.21%. Experiment 2 explores the broiler hunger Training - Determination of concentrated feed digestion and metabolism of nutrients in feed digestion and metabolism rate method, the choice of 30 d similar body weight, feed healthy normal Arbor Acres broilers 100 were randomly divided into A , B two groups of 10 replicates of five. A group of first through 5 d-by-day hunger shorten feeding time training, and then for 60 min feeding time hunger training, B directly with 60 min of feeding time hunger training. The results showed that, after appropriate Hunger - concentrated feed training (duration 5 d above), within 60 min broiler feed can complete the general metabolism of the amount of feed required for testing. Test 3 single factor randomized trial design, selection of similar weight training through hunger, feed healthy normal Arbor Acres broilers 50, were randomly divided into 10 groups, each five replicates a chicken , five test diets, wherein xylanase levels were 2.00%, 3.50%, 5.00%, 6.50%, 8.00%, 1 to 5 groups of dietary no enzyme control, dietary group of 6 to 10 added xylanase preparation process group. The results showed that: (1) When the diet xylan content of less than 5.00%, the xylanase preparation can improve dry matter, calcium and phosphorus metabolic rate, increase energy and nitrogen impact trends. Xylan level of 2.00%, the xylanase preparation improves broilers dry, calcium and phosphorus apparent metabolic rate were 9.09% (P lt; 0.01), 15.02% (P lt; 0.05) , 18.52% (P lt; 0.01), for broilers dry matter, calcium and phosphorus true metabolic rate, enzyme were increased 3.76% (P lt; 0.05), 16.32% (P lt; 0.01), 21.82% (P lt; 0.01); dietary xylan level of 3.50%, the xylanase enzyme supplementation on dry matter true metabolic rate increases 4.42% (P lt; 0.05), phosphorus apparent metabolic rate increased 19.53% (P lt; 0.01), calcium apparent metabolic rate increased by 14.44% (P lt; 0.05), but also improves the metabolic rate of nitrogen true 31.85% (P lt; 0.01). When dietary xylan content higher than 5.00%, the added enzyme supplementation on metabolism of various nutrients were not significantly affected. (2) the level of dietary xylanase between 2.00% ~ 8.00%, the dry xylan content and broilers, phosphorus, and the true metabolic rate of energy there is a significant negative correlation with the increase in the content of xylan , decreased metabolic rate, regression equations were y =-119.4000x 90.0580 (R2 = 0.8841, P = 0.0174), y =-237.0000x 68.4160 (R2 = 0.8138, P = 0.0362) and y =-159.1333x 92.6567 (R2 = 0.9934, P = 0.0002). (3) In the xylanase preparation diet, xylan content of dry matter and phosphorus broilers only true metabolic rate there was a significant negative linear correlation, regression equations were y =-195.0667x 95.1793 (R2 = 0.8833, P = 0.0176), y =-547.3333x 90.9787 (R2 = 0.7991, P = 0.0408), but because of xylan content higher than 5.00%, the addition of this enzyme supplementation on dry matter, phosphorus really no significant metabolic rate impact, therefore two equations Scope of dietary xylan level of 2.00% ~ 5.00%.
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