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Chitosan applied to the seed coating agent process and the use of forestry as a soil conditioner, a pest control agent, and herbicides controlled release agents. And Tenebrio is an easier feeding insects are omnivorous, feeding method is simple, high protein content, commonly used for feed, food additives and direct food. Its factory extracted protein when extracting protein, usually to slough abandoned use of the yellow mealworm extract chitosan transformation can be discarded waste into treasure, orthogonal experimental trials, trying to find a sets the optimum conditions of extraction of Tenebrio slough chitosan. The tree seed Seed Coating chitosan application and extraction Tenebrio slough as Seed Coating resistant additives initial exploration, the main conclusions are as follows: (1) Research Seed Coating tree breeding insect resistance and to promote the growth and development role, in order to determine the feasibility of forestry. Huang Jing, Tibetan cypress, Pinus armandii, Japanese cedar, Chinese pine, Amorpha, locust, Masson pine seed experiment artificial coating treatment, placed in petri dishes sprouting and growing observed at room temperature, and in Kangding County the outdoor insect resistance experiments, the effect of comparison between the four kinds of seed coating agent. The results showed that: 22% Thiram pentanol BH101 seed coating agent to promote seed growth and development is the best, and insect resistance package clothing seed germination rate are greatly be enhanced. (2) The test results show that the impact of different process conditions Tenebrio slough chitosan extracted: off protein concentration of sodium hydroxide, sodium hydroxide concentration deacetylation, the role of time and temperature on the chitosan extraction rate affects both the quality and appearance. Tenebrio slough chitin / chitosan optimum extraction conditions and different conditions product index the degree obtained by orthogonal experiment. The optimum conditions for first with 6% sodium hydroxide at a temperature of 85 ℃ case processing 3.5h, and then use the reaction temperature of 90 ° C, 40% sodium hydroxide concentration, reaction time 14 h, chitosan harvest rate up to about 17%, the deacetylase greater than 75%. Chitosan products yield the most significant effect of the factor is the concentration of alkali, followed by the reaction time, the reaction temperature is again. (3) the use of different concentrations (0.1%, 0.2% and 0.3% concentration) chitosan solution soaking and seedling pine, Luan tree, acacia, acacia, corn, wheat, explore chitosan on seed germination and Physiological and biochemical characteristics affect the test results show that the various sub germination rate, germination potential, seedling growth and vigor index were significantly higher than the control; growth chitosan solution sprayed in leaves, chlorophyll, soluble protein content significantly improve; memory and chitosan in a certain range, the best treatment effect in the concentration effect, concentration O.2%. (4) in the test of Seed Coating film-forming agent chosen, through the selection of the waxy corn starch, chitosan and carboxymethyl cellulose sodium as a film-forming agent, and measuring the pH value, the deposition time, and the shedding rate in order to determine the good or bad. The results show that the chitosan has a good film forming properties, under natural conditions, the coating can be quickly cured film, and firmly adhered to the surface of the seeds does not fall off, no adhesion, not block.
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