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Inhibitory Effect of Probiotics on Bacterial Wilt of Eucalyptus

Author: DengZhaoFeng
Tutor: HuangBaoLing
School: Guangxi University
Course: Forest cultivation
Keywords: Probiotics Eucalyptus Bacterial wilt Prevention effect
CLC: S763.7
Type: Master's thesis
Year: 2008
Downloads: 99
Quote: 0
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Abstract


Eucalyptus (Eucalyptus spp.) Is the general term for Myrtaceae Eucalyptus, their origin to Australia and a few islands of Indonesia and nearby. Because of its strong resistance, tolerance to drought and barren, wide adaptability, planting, sprouting ability, fast-growing high-yield and other characteristics, to become China's most important commercial forest of fast-growing exotic species, the large-scale cultivation in southern China. But encountered many problems in a large area of ??the development of the fast growing forest of eucalyptus, which Wilt Disease in disease is more serious. Therefore, a reasonable test to find the right approach to combating Wilt has a very important significance. This paper studies probiotics for the purpose of of Wilt prevention effect, made a comprehensive study probiotics on the growth effect of eucalyptus and control effect. The preliminary test results are as follows: 1. Sampling from the wild separation filter out four virulence strains of Ralstonia solanacearum (Ⅴ -1-1, IV -1-1 Ⅴ -1-2, Ⅲ -2-2 ), the virulence of the strain Ⅴ -1-1 strongest. The puffball fungi isolated 24 strains, can be seen by the test has a different degree of antagonism to R. solanacearum. Fixing bacteria in this test N-3, the PSB P15, the solution potassium strain K4-3 and K14 wilt fungus has a certain amount of antagonism, but further tests to verify. 2. The test 10 tinctorius fungi growth of Eucalyptus seedlings have a role in promoting different degrees. Three indicators measuring height, diameter and biomass in the three months after the inoculation, where height ratio was increased by 14.5% -68.1% 8.1% -49.7%; seedlings ground diameter were increased; seedling biomass (dry weight) than were increased by 5.3% -58.8%. Eucalyptus seedling biomass, height growth, the amount of diameter growth three indicators to select superior strains of the eucalyptus growth effect good that after a comprehensive comparison of strains 1-6,2-1,2-3,5-6 as the excellent Strains. The test 11 probiotic combination with different degrees of growth of Eucalyptus seedlings role. Three indicators measuring height, diameter and biomass in the three months after the inoculation height were increased by 21.7% -62.7% 13.8% -48.8%; seedlings ground diameter were increased; seedling biomass (dry weight) than were increased by 2.3% -88.3%. Eucalyptus seedlings biomass, height growth and diameter growth of three indicators to select a the excellent strain combination of good growth effect of Eucalyptus, comprehensive comparison strain combinations of Z-3, Z-5, Z-8, Z -4, Z-2, Z-11 for fine strain combination. Mycorrhizal seedlings were inoculated with different the puffball fungi and probiotic combinations to form in artificially inoculated R. solanacearum conditions, can reduce the mortality and disease index of bacterial wilt, or postpone the occurrence of bacterial wilt period. In addition to the individual strains, most strains reduce the role of Wilt, indeed some effect. The the seedlings inoculated puffball fungi earliest days of the onset generally delayed 2-9d, 6.7% -40% lower mortality rate than the control seedlings; disease index 9.1% -40.8% lower than that of the control seedlings, which strains 5-6,1-6 ,6-1 ,2-1 ,4-1 disease prevention is better; seedlings inoculated with probiotic combination the earliest days of the onset generally delayed 1-9d, 20% -46.6% lower mortality rate than the control seedlings; disease index than the control the seedlings reduced by 15% -46.6%, and wherein to the composition of the strains Z-4, Z-3, Z-8, Z-5 and Z-6, Z-9 prophylactic better. Therefore, the next step is validated by field trials using eucalyptus mycorrhizal Planting will allow current bacterial wilt Injury has increased year by year the status of the area and the distribution range of partially improved. This method is currently the fight against one of the effective and feasible method Wilt.

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CLC: > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > All kinds of tree pests and diseases and their prevention
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