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Research on Daunorbic Nanoparticles Permeation Through Blood Brain Barrier

Author: LiuMeiZhou
Tutor: PengJian
School: Central South University
Course: Surgery
Keywords: daunorubicin nanoparticle blood brain barrier
CLC: R96
Type: Master's thesis
Year: 2007
Downloads: 134
Quote: 1
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Abstract


Objective: To prepare nanoparticle drug carriers for daunorbic, and to simulate a simple in-vitro cell model of blood brain barrier, as well as to provide evidence on clinical application of daunorbic nanoparticles.Methods:①Polybutylcyano acrylate was selected to be drug carrier, and daunorubicin polybutylcyano acrylate nanoparticles were prepared using emulsifi-polymerization. DAM-PBCA-NP, examined with single factor experiment and equality test, was analyzed and evaluated on multi factors, in terms of drug load of daunorubicin, coating rate, and average particle diameter, for optimizing preparation of daunorubicin nanoparticle.②SD rats were selected as the experimental material. Micro-blood vessels were attained using two-step filtration. Assimilated by collagen enzyme and centrifuged with low-molecular dextran, micro-blood vessel endothelium cells were gained. Cell culture base was changed 4 hours after inoculation. With inversion microscope, cellular purification was immunohistochemically determined byⅧF-Ag, and cellular activation was examined using MTT method. The second generation micro-blood vessel endothelium cells were inoculated inner Transwell and cultured for 1 week. Rate of permeation through blood brain barrier was determined by DAM-PBCA-NP distribution in the in-vitro model.Results:①DAM-PBCA-NP solution presented red with optimized preparation. Particle shape was round and smooth, well distributed, with an average diameter of (82±3) nm. Its coating rate reached (92.65±1.87)%, and drug load was (68.52±1.56)%.②Micro-blood vessel endothelium cells gained with separated culture approach had multi-angle shape with monolayer growth. Immunohistochemical test showed positive, and cellular purification reached 90%.③Rate of permeation through blood brain barrier was (70.15±1.23) %.Discussion:①The optimized preparation of DAM-PBCA-NP satis fied the needs in the present study, which could be considered the best preparation.②The two-step method for attaining rat micro-blood vess el endothelium cells is easy to approach, and the high cellular purification was suitable for BBB model.③Rate of permeation through blood brain barrier was (70.15±1.23) %, which has provided clinical evidence for further in-vivo experiments.

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