Dissertation > Excellent graduate degree dissertation topics show

The Influence of MCP-1 to Photoreceptor Apoptosis in Rat Experimental Retinal Detachment

Author: MiaoGangYong
Tutor: TangLuoSheng
School: Central South University
Course: Ophthalmology
Keywords: MCP-1 experimental retinal detachment TUNEL photoreceptor apoptosis
CLC: R774.1
Type: Master's thesis
Year: 2008
Downloads: 30
Quote: 1
Read: Download Dissertation

Abstract


Objective:(1)To explore Monocyte chemoattractant protein-1 expresses in rat experimental retinal detachment at different times;the relationship between MCP-1 and photoreceptor apoptosis in retinal detachment.(2)To explore blocking the expression of MCP-1 in retinal detachment can diminish photoreceptor apoptosis.Methods:46 Brown Norway rats were involved in this study.(1)37 rats were builded the retinal detachment model in the right eye,and the left eye as control.Except 7 rats which were not fit to the standard model(include death, endophthalmitis and retinal hemorrhage).The left 30 rats devided into 5 groups according force-managed times(1h、6h、12h、24h、72h).Then rats were killed and the eyes were enclutered.The expression of MCP-1 in retinal detachment eyes were assessed by immunohistochemical method and cell death were quantified by TUNEL assay.Analysis the relatinship between MCP-1 and TUNEL in detached retinal by statistical software.(2)9 rats were builded the retinal detachment model both of the eyes.We injected rat anti-MCP-1 IgG subretinally during RD induction in the right eyes and PBS in the left eyes. Except 3 rats which were not fit to the standard model(include death,endophthalmitis and retinal hemorrhage).Cell death were quantified by TUNEL assay 72h after RD,since at that time the number of TUNEL-positive cells in the ONL is at the peak.Resuits(1)Immunohistochemical analysis:In normal retinal tissue,the number of MCP-1 positive cells was 3.64±1.02 under microscopy visual field.MCP-1 positive cells was 10.50±2.82、11.13±3.31、25.23±5.56、37.23±7.15、205.83±15.23 in detached retinal at 1h、6h、12h、24h、72h under microscopy visual field.Compare the expression of MCP-1 at different times of RD,every two groups had statistical variation,except the 1h and 6h group.(2)TUNEL assey:In normal retinal tissue,the number of TUNEL positive cells in ONL was 2.16±1.33/mm~2.TUNEL positive cells in ONL was 15.30±3.21/mm~2;25.11±5.31/mm~2;41.32±7.56/mm~2; 80.23±10.15/mm~2;425.83±25.23/mm~2 in detached retinal at 1h、6h、12h、24h、72h.(3)MCP-1 and TUNEL positive cell began to express remarkably after 1h of retinal detachment,then increased follow time,got the peak at 72h.The relationship between MCP-1 and TUNEL positive cell is positive correlation(r=0.905).(4)TUNEL positive cells in MCP-1 blocking group and PBS group after 72h of retinal detachment were 160.24±10.56/mm~2 and 430.15±30.42/mm~2 in ONL.There was statistical variation between MCP-1 blocking group and RD group(p＜0.05),no statistical variance between RD group and PBS group(p＞0.05).Conclution:(1)MCP-1 began to express after 1h of rat retinal detachment,then increased follow time,got the peak at 72h.(2)There is positive correlation between MCP-1 and TUNEL positive cell.(3)Blocking the expression of MCP-1 in detached retinal can diminish photoreceptor apoptosis.

Related Dissertations

  1. The Research Report about the Storing Quality of Jiro Persimmon Treated by 1-MCP Combined with Nano-Packaging,S665.2
  2. Analysis of Nanguo Pear Aroma Components and Its Variation during Storage,S661.2
  3. The Expression and Significance of Serum MIP-1β and MCP-1in Patients with Rheumatoid Athritis,R593.22
  4. Rhizoma Coptidis Inhibits LPS-induced MCP-1 Production in Murine Macrophages,R285
  5. MCP face electron reflection on the impact of image intensifier resolution,TN144
  6. Correlation between the Expression of High Mobility Group Box 1 and it Ligand Receptor for Advanced Glycation Endproducts in Lupus Nephritis,R593.242
  7. Experimental Study on the Effect of Tubulointerstitial Injur of IGA Nephropathy in Rat with Shen-Yan-Zhi-Xue Pill,R285
  8. Semi-continuous function into outer space,O189.1
  9. Study on the Metabolic Characteristic of Physiological and Biochemical in Fresh-cut Carrot,TS255.3
  10. Effects of Maturity and 1-MCP Treatment on Fruit Quality and Browing of Banana-plum During Cold Stroage,S662.3
  11. ~ (18) F-SFB-Annexin B1 probe Experimental evaluation of apoptosis,R817.4
  12. Study on Gene Therapy of SiRNA Targeted at Telomerase of Hepatocellular Carcinoma Transduced by Lentivirus in Vitro,R735.7
  13. Research on Expression of Chemokine MCP-1 and IL-8 in Peripheral Blood of Gastric Cancer Patients,R735.2
  14. Functional Evaluation of MCP-1 to Detect the Obstructive Hydronephrosis,R692.2
  15. Intelectin airway inflammation in asthma study the role of,R562.25
  16. Acupuncture expression of chemotactic factor-1 in myocardial tissue of rats of experimental diabetic cardiomyopathy,R245
  17. Treatment of cooling Xiaofengsan clinical observation and subacute eczema on IL-8, MCP-1 Preliminary impact,R275
  18. Pharmacokinetic Study of Antioxidants Lipoic Acid in Guinea Pig Inner Ear and Its Inhibitory Effects on Apoptosis after Acute Acoustic Trauma,R96
  19. Effects of Propofol on Cell Viability and Apoptosis in Developing Cortical Neurons,R965
  20. The Effect of Resveratrol on Expression of Apoptosis-related Gene PDCD5 in BGC-823 Cell,R735.2
  21. SLC30A8 and Monocyte Chemoattractant Protein 1 Polymorphism and Susceptibility to Type 2 Diabetes,R587.1

CLC: > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease
© 2012 www.DissertationTopic.Net  Mobile