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Study on the p53 and K-ras Gene Mutations in Gastric Carcinomas

Author: ChenHuiJuan
Tutor: ChenHanChun；ZhangDianZheng
School: Central South University
Course: Biochemistry and Molecular Biology
Keywords: Gastric cancer p53 K-ras Gene mutation SSCP
CLC: R735.2
Type: Master's thesis
Year: 2008
Downloads: 168
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Abstract


Objective: To detect tumor suppressor gene p53 and the original oncogene K -ras mutations in the the China Hunan crowd gastric cancer patients and the normal population , analysis of the impact of the tumor suppressor gene p53 and the proto-oncogene K-ras gene mutation in Hunan crowd gastric . Methods: Case - control analysis ( control group, 129 cases of gastric cancer group 123 patients) was extracted from peripheral blood genomic DNA using the polymerase chain reaction - single strand conformation polymorphism (PCR-SSCP) analysis combined with silver staining techniques and sequencing to detect mutations in gastric cancer patients with p53 and K-ras gene . Results: p53 gene mutations in gastric cancer patients in the Hunan province was 29.3% , mainly occurs in exon 5 region mutations mainly G: C → A: T (15/36, 41.7%), the mutation site was decentralized distribution. p53 gene mutations in the patients' gender , age , history of smoking and drinking history and other factors unrelated (P> 0.05), with the degree of tumor differentiation ( P = 0.006 ) ; higher mutation rate of the p53 gene in patients with lymph node metastasis ( 42.3% ) . K-ras gene mutation rate was 9.76% , and the 12 codon mutations . The type of mutation G → A conversion . Logistic single factor and multivariate analysis results show that : the K - ras gene mutations in the patients' gender , age, history of smoking and drinking history , lymph node metastasis and tumor differentiation factors unrelated ( P > 0.05 ) . Two cases of p53 and K-ras gene mutation found in this study , statistical analysis showed no correlation ( P > 0.05 ) between the two mutations . Conclusion: The China Hunan crowd , p53 gene K- ras gene mutations in gastric cancer is closely related . The two gene mutations there is no correlation . Gastric p53 gene mutation may indicate a higher degree of tumor differentiation and may promote lymph node metastasis .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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