Dissertation > Excellent graduate degree dissertation topics show

The Role of Raf Kinase Inhibitor Protein in Nasopharyngeal Carcinoma Metastasis

Author: OuYangGuoLiang
Tutor: XiaoZhiQiang
School: Central South University
Course: Pathology and Pathophysiology
Keywords: Nasopharyngeal Invasion and metastasis Proteomics Raf kinase inhibitor protein
CLC: R739.63
Type: Master's thesis
Year: 2007
Downloads: 87
Quote: 0
Read: Download Dissertation

Abstract


Background: NPC in southern China and Southeast Asia is one of the most common malignancies, early metastasis is one of the most important features of NPC is the main reason for treatment failure in patients, but nasopharyngeal transfer mechanism is unclear. Raf kinase inhibitor protein (RKIP) is a highly conserved, ubiquitously expressed, with a variety of physiological and pathological functions of proteins, recent studies have shown that, RKIP with prostate cancer and metastasis of breast cancer, may be a new tumor metastasis suppressor gene. Methods: Using proteomics separation, identification nasopharyngeal carcinoma and normal nasopharyngeal mucosa differentially expressed proteins. To differences in protein RKIP as the research object, using Western blot analysis RKIP in NPC cells with different metastatic potential 5-8F (highly metastatic) and 6-10B (not transferred) cell expression levels detected by immunohistochemistry in RKIP normal nasopharyngeal mucosa, nasopharyngeal carcinoma and cervical lymph node metastasis tissue level; using lipofectamine method sense and antisense nucleic RKIP expression plasmid pcDNA3.1 ()-ssRKIP and pcDNA3.1 (- )-asRKIP its corresponding blank vector pcDNA3.1 () and pcDNA3.1 (-) were transfected into 5-8F and 6-10B cells and establish the corresponding stably transfected cell lines. In stably transfected cell lines as a sample, Transwell cell migration and invasion assay RKIP expression level changes on nasopharyngeal carcinoma cell migration and invasion ability of; MTT, flow cytometry and soft agar colony formation assay RKIP expression level changes on the nose pharyngeal cancer cell proliferation, cell cycle distribution and growth of anchorage-independent. Results: (1) establishment of nasopharyngeal carcinoma and normal nasopharyngeal mucosa of 2-DE maps, identified 31 differentially expressed proteins, mass spectrometry identified 19 differentially expressed proteins, such as protein in nasopharyngeal RKIP organization down; (2) RKIP in highly metastatic cell lines 5-8F expression levels than those without metastatic potential of 6-10B cells, lymph node metastasis in nasopharyngeal carcinoma levels below the primary nasopharyngeal carcinoma ; (3) to establish a RKIP upregulation of 5-8F cell lines and RKIP downregulation of 6-10B cells; (4) increase RKIP in 5-8F cells can significantly reduce the level of invasion and migration capabilities, reduced RKIP In the 6-10B cells can significantly enhance the expression level of migration and invasion; (5) raised RKIP 5-8F cells in the expression levels can inhibit the proliferation and parking-independent growth, and cause the occurrence of G 0 / G 1 of the block, down RKIP 6-10B cells in the expression level to promote their proliferation and parking-independent growth, and accelerated over the 6-10B cells G < sub> 0 / G 1 phase. Conclusions: RKIP inhibits nasopharyngeal carcinoma cell invasion and migration, proliferation and anchorage-independent growth effect, RKIP may be nasopharyngeal carcinoma metastasis suppressor gene. The first study found that RKIP may be nasopharyngeal carcinoma metastasis suppressor gene, not only helps to reveal the mechanism of transfer of nasopharyngeal carcinoma, but also for targeted inhibition of metastasis of nasopharyngeal provide an experimental basis.

Related Dissertations

  1. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  2. Proteomic Research of Rice Plasma Membrane Protein under Blast Stress,S511
  3. Salt-Tolerance Evaluation of Brassica Napus Germplasm in the Bud and Seeding and Establishment of the Two-Dimensional Electrophoresis System,S565.4
  4. The Study of Clinical Prognostic Factors in Nasopharyngeal Carcinoma after Intensity Modulated Radiation Therapy,R739.63
  5. Research for the Expressive Correlation of EBERs and BMI-1 in Nasopharyngeal Carcinoma,R739.63
  6. Quantiative Proteomics of Liver Immortalized Cell Line Chang Liver and Liver Cancer Cell Line HepG2,R735.7
  7. The Extraction and Application of Endogenous Serum Phosphopeptides from Lung Cancer Patients,R734.2
  8. Diazoxide post-processing in the rat heart mitochondria comparative proteomics research,R965
  9. Study on Biological Characteristics and Formation Mechanism of Flower Color Chimera in Chrysanthemum,S682.11
  10. The Comparative Analysis of Radiotherapeutic Prognosis of Nasopharyngeal Carcinoma According to Chinese 1992, 2008 and UICC2010 Staging Systems,R739.63
  11. Clinical Study of the Expression of Raf Kinase Inhibitor Protein and VEGF-C in Cervical Carcinoma,R737.33
  12. The Effect of MACC1 on Invasion and Metastasis in Prostate Cancer Cell Lines (1Ee8/2B4),R737.25
  13. The Expression of Hexokinase Ⅱ in Nasopharyngeal Carcinoma,R739.63
  14. The E-cadherin Promoter-160 C/A Polymorphism and Nasopharyngeal Carcinoma in a Chinese Population,R739.63
  15. Studies on in Vitro Flowering and Proteomics in Tagetes Patula L.,S681.9
  16. The Preminary Study about the Effect of Rosiglitazone on Proliferation Inhibit and Radiosensitivity of Human Nasopharyngeal Carcinoma Cell Line CNE-2,R739.63
  17. The Research of Calcium, Phosphorus Concentrations in Saliva of Patients with Nasopharyngeal Carcinoma before and after Radiation Therapy,R739.63
  18. Related Risk Factors to Caries after Nasopharyngeal Carcinoma Radiotherapy,R739.63
  19. To Study the Relationship Between Expression of Annexin A5 in Nasopharyngeal Carcinoma,R739.6
  20. Study of the Expression and Significance of Twist in Nasopharyngeal Carcinoma,R739.63
  21. Antitumor Effect of Bleomycin-conjugated Single Domain Antibody Against Human Nasopharyngeal Carcinoma,R739.6

CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
© 2012 www.DissertationTopic.Net  Mobile