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A549 cells type Ⅱ basic parameters photodynamic reaction analysis and quantitative application of mathematical models

Author: ZhangJie
Tutor: WangChangLi
School: Tianjin Medical University
Course: Oncology
Keywords: Photodynamic therapy Lung cancer Cell Cycle Apoptosis Mathematical model
CLC: R734.2
Type: Master's thesis
Year: 2008
Downloads: 62
Quote: 0
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Abstract


Objective: To study the role for photodynamic killing effects of lung cancer cell line A549 basic conditions and parameters , applied mathematics photodynamic quantitative models to simulate the effect of various parameters to determine the reasonableness of the model to further damage to cells and extract simulation results directly photochemical dynamic parameters . Materials and Methods: 630nm high-power semiconductor lasers, Germany hematoporphyrin derivative photosensitizers for photodynamic reaction photosan3 , lung adenocarcinoma cell line A549; different measured by MTT assay plates , different photosensitizer concentrations and different laser power cells after PDT OD 492 values ??and calculate the cell survival rate ; flow cytometry determination PDT effect on lung cancer cell line A549 cell cycle and apoptosis rate determination ; application created photodynamic Learn general mathematical model of the experimental results of cell simulation and interpretation of reaction parameters . Results: black clear bottom 96-well cell experiments PDT cells obtained residual ratio significantly higher than the ordinary transparent plate 96 is high ; the same power and energy density, the cell survival rate in a certain range with the photosensitizer concentration decreased , However, the concentration of the photosensitizer 4mg / l or less, and 20mg / l or more, not trend ; same photosensitizer concentration and energy density, the cell survival rate in a range of optical power increases with the decrease , but the power is less than 5mW and more than 50mW when the trend is not obvious ; flow cytometry to detect cell cycle PDT group found that the proportion of cells in G0/G1 phase increased , the growth occurred in the G0/G1 phase standstill , S phase and G2 / M phase cells decreased ; PDT apoptosis rate significantly higher ; mathematical model of the hypoxia and oxygen under conditions of photon density of different molecules participate in the reaction rate of change with time and the amount of change in the incident photons and intracellular receptor content between decay time . Conclusion : The bottom black 96-well plates for clarification PDT cell experiments , compared with ordinary transparent 96 -well plate can significantly reduce experimental error ; photosensitizer concentration in a certain range of the laser power and lung cancer cell line A549 residual rate significantly decreased, but this range is too large or too small will affect this trend ; PDT on lung cancer cell line A549 arrest occurs mainly in the G1 phase ; cell killing mechanisms of apoptosis and necrosis ; mathematical model can simulate and interpret trends in cell experiments .

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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