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Objective: Lung cancer is one of the most common malignant tumor of the people's health hazards is great, but its pathogenesis has not yet been elucidated. A lot of research and experiments show that the incidence of lung cancer, the development and the process of transferring the regulation of various genes, tumor angiogenesis substances and morphological basis for tumor growth and metastasis. The early 1970s American scholar Flokman first proposed tumor growth depends on angiogenesis, to early 90s further improve the Flokman the doctrine. Tumor growth can not be separated from the regeneration of the nutrient vessels, tumor angiogenesis, tumor growth and metastasis morphological basis, significantly affect the prognosis. Vascular endothelial growth factor (vascular endothelial growth facto-r, VEGF) is a major lung cancer angiogenesis is one of the positive regulatory factor, high specificity, is the main factor that stimulates tumor growth, with tumor growth, invasion and metastasis. Relevance. Microvascular density (micro vascula density, MVD), is considered to be the best reaction tumor angiogenesis indicators. CD34 are present in the vascular endothelial cells on one antigen, its molecule has been purified and cloned is a different factor Ⅷ related antigen protein. Studies have shown that CD34 immunohistochemical staining vascular endothelial cells more sensitive than factor VIII related antigen specificity also stronger. nm-23 gene is the Steeg equal to 1988 isolated from a cDNA gene in murine K-1735 melanoma. For VEGF and lung cancer, but the lung cancer VEGF, MVD, nm-23 expression and correlation with clinical stage, pathological type of relations is still at a relatively blank stage. In this study, immunohistochemical method detection nm-23 and VEGF in lung cancer and determination of microvessel density (MVD) CD34 monoclonal antibody markers to investigate the expression of lung cancer the angiogenesis and clinicopathologic factors relating to the relationship and in lung cancer role in the development occurred. Reveals three in the development of lung cancer and prognosis nm-23 expression and VEGF, MVD expression exists, you can provide the basis for the monitoring and treatment of lung cancer, combined with three other lung products expression of lung cancer clinical stage, pathological type, provide the theoretical basis and foundation for the development of lung cancer research and treatment. : Collected after surgical removal of pathologically confirmed 62 cases of lung cancer cases, all patients without radiotherapy or chemotherapy, record each patient's gender, age, swollen size, histological type, pathological stage clinical data, using immunohistochemical SP method detected specimens VEGF, NM-23 and MVD expression. Select no necrosis of cancer, 30 cases of adjacent tissues as a negative control, tissue from the lesions cut end more than 5cm. Positive standard: each slice select a representative sample of five high power field were observed count per field counting 100 tumor cells. Positive cells appear brown particles, using a semi-quantitative integration method, respectively, were graded according to the degree of staining and the percentage of stained cells ratings, to determine the extent of the positive and according to the product of two terms. The degree of staining: Basic colored stars 0 with pale stars 1, coloring medium in Bad 2 stars, 3 stars deep coloring; coloring cells accounted for the percentage of cell count: ≤ 5% 0 stars, ≤ 6% ~ 25% ≤ 26% ~ 50% 2 stars 1 stars ≥ 51% for the 3 stars. The average degree of coloration of each slice score score and the average percentage of colored cells multiply each of their final score: ≤ 1 is divided into the negative (-), 2 to 3 divided into positive () ≥ 4 strongly positive () Applied Statistics learning software SPSS for Windows 15.0, chi-square test and Spearman correlation analysis method to analyze the relationship between the three NSCLC and three. Results: 1.62 cases of lung cancer specimens VEGF nm-23 and MVD positive expression rate was 61.3% (38/62), 51.6% (32/62) and 61.3% (38/62), 30 cases of adjacent organizations VEGF, nm-23 and MVD expression rate was 16.7% (5/30), 23.3% (7/30) and 20% (6/30), respectively, there were significant differences (P lt; 0.05) . VEGF, NM-23 and MVD in the patient's gender, age and tumor size, histological type (P GT; 0.05) VEGF, NM-23 and MVD expression of the degree of differentiation of lung cancer, clinical stage and lymph node metastasis significant difference (P lt; 0.05). 4 MVD VEGF positive expression of tissue high expression rate was 76.3% (29/38), both positive expression was positively correlated (r = 0.388, P lt; 0.01) VEGF positive expression of tissue nm-23 expression was 36.8% (14/38), both positive expression was negatively correlated (r = -0.372, P lt; 0.01) 6 nm-23 expression and MVD expression was no correlation (P gt; 0.05) Conclusion: 1 nm-23 positioning in the cytoplasm, the positioning of VEGF in the cytoplasm, and CD34 mainly located in the cell membrane. VEGF, NM-23 and MVD expression with sex, age and tumor size, pathological type and degree of differentiation of lung cancer, clinical stage and lymph node metastasis. VEGF positive expression and MVD expression positively correlated with VEGF positive expression was negatively correlated with nm-23 positive.
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