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Experimental Study of Inhibitory Effect of Growth on Rats Bladder Tumor and Apoptosis-Induced by Allicin

Author: ZhouShengCai
Tutor: TangWei
School: Chongqing Medical University
Course: Surgery
Keywords: Allicin Bladder Cancer Apoptosis Reverse transcription polymerase chain reaction Immunohistochemistry caspase-3
CLC: R737.14
Type: Master's thesis
Year: 2008
Downloads: 147
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Abstract

Objective: To explore allicin on the treatment of bladder cancer, reveals the relationship between apoptosis and further found that the in vivo anti-tumor potential mechanism of action for the treatment of bladder cancer find a new drug. Methods: a rat bladder tumor model: with N-methyl nitrosourea bladder tumor were established rat model. (2) the 60 tumor-bearing rats were randomly divided into four groups, each 15, were saline, cisplatin group, low concentration of allicin group, high concentrations of allicin group. Route of administration: intraperitoneal injection. Every other day, a total of seven times. During the state of life of rats and weight changes, rats were killed after injection, the detection of bladder total weight changes, HE staining of rats bladder tumor histopathological changes. 3. TUNEL method to detect the apoptosis of bladder tumor tissue, RT-PCR and immunohistochemistry were used to detect the rat bladder tumor tissue caspase-3 mRNA and protein expression, revealing allicin-induced apoptosis of bladder cancer possible mechanisms. Results 1.N-MNU (MUN) intravesical bladder tumor induced histopathological changes MNU l2 weeks after the first infusion were sacrificed five tumor-bearing rats, anatomical cut the bladder, the bladder wall found thickened, pale, cauliflower-like formation of new biotechnology, the vast majority of multiple tumors, severe cases have reached the full extent of the bladder. Week 12 bladder tumors HE staining showed the typical performance of bladder transitional cell carcinoma with focal submucosal, myometrial invasion. Other organs were normal. 2 inhibitory effect of allicin before and after administration of tumor-bearing rats showed body weight saline group, the low concentration of allicin group rat body weight, administered before and after comparison, the differences were not statistically significant (p gt; 0.05) High concentrations of allicin group rats, body weight was significantly higher than in rats after administration prior to administration, a significant difference (P lt; 0.05), and tumor-bearing rats after administration of a good appetite, normal activities, shiny fur . And cisplatin in tumor-bearing rats significantly reduced body weight after administration, and prior to administration and other groups, the differences were statistically significant (P lt; 0.05), and showed significant loss of appetite, activity slow, dirty dark coat. High concentration of allicin rats was significantly lower than the total weight of the bladder and a low concentration saline group allicin group, the difference was significantly (p lt; 0.05); low concentration and high concentration of allicin group allicin group, the difference was significantly (p lt; 0.05); cisplatin group with low concentrations of allicin group showed no significant difference (p gt; 0.05). Saline group before and after treatment of bladder tumor histopathological changes little, tumor cell growth activity, without apparent death situation, and cisplatin group and different concentrations of allicin group, there are different degrees of bladder tumor tissue necrosis. Light microscope, tumor cell apoptosis was difficult to observe. 3 Allicin induced apoptosis of bladder cancer and caspase-3 mRNA, protein expression detected by TUNEL tumor tissue of rats nuclei are different degrees of brown staining. Compared with the saline group, the other AI in each experimental group were significantly higher than the saline group (P lt; 0.05). And with the high concentration of allicin group cisplatin group, the low concentration of allicin group, apoptosis is more obvious, the difference was statistically significant (P lt; 0.05), and with the increase of the concentration of allicin, AI values ??increased. This study found that allicin can at the mRNA level to promote bladder tumor tissue upregulation of caspase-3, and the expression level increases with the concentration rises. Immunohistochemical SP method to detect caspase-3 protein in rat bladder tumor tissue levels observed under an optical microscope, caspase-3 protein positive product was brown in some tumor cell membrane in the cytoplasm and some . And found that allicin can increase caspase-3 protein in bladder tumor tissue expression levels. Conclusions: 1. MNU intravesical bladder tumors in rats were induced models, methods and reliable tumor formation rate, is a good way to establish bladder tumor model. 2 allicin not only security and reliable, with the chemotherapy drug cisplatin compared to drug side effects, and bladder tumors in rats have significant anti-tumor effect. 3 allicin apoptosis by upregulating the expression of caspase-3 gene in vivo induction of apoptosis in bladder tumor tissue.

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