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Influence of Intravenous Administration of Bone Marrow Mesenchymal Stem Cells on Expression of VEGF、bFGF and Regeneration of Blood Vessel after Spinal Cord Injury in Rats

Author: JiaXiaoLi
Tutor: ChenShaoQiang
School: Fujian Medical
Course: Human Anatomy and Embryology
Keywords: Bone marrow mesenchymal stem cells Spinal cord injury Vein graft
CLC: R651.2
Type: Master's thesis
Year: 2008
Downloads: 156
Quote: 0
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Abstract


Objective: marker fluorescent bone marrow mesenchymal stem cells (bone mesenchymal stem cells, the body of the rat model of MSCs vein grafts to spinal cord injury (spinal cord injury, SCI), observation of MSCs in the survival of the damage zone and vascular endothelial growth factor (vascular endothelial growth factor, VEGF) and basic fibroblast growth factor (basic fibroblast growth factor, bFGF) expression and angiogenesis circumstances, the MSCs transplantation for the treatment of SCI and its mechanism to provide experimental basis. methods: 1. vitro isolated and cultured MSCs by flow cytometry detected surface markers. 2 Animal groups: 42 adult healthy SD male rats (body weight 200g-250g) were randomly divided into 7 groups, each group of six, three groups MSCs transplantation group; 3 groups for the control group Allen device; 1 for the normal group. preparation of the spinal cord injury model: homemade improved impact T12 segment of spinal cord and traumatic spinal cord paraplegia model. 4. carboxy-fluorescein diacetate succinimidyl ester (carboxyfluorescein succinimidyl ester, CFSE) vitro labeling MSCs5. injection of the same amount of phosphate-buffered saline (phosphate-buffered saline, PBS) the MSCs transplantation MSCs group 6: after 3 days by intravenous injection of MSCs control group 6: 6. drawn and detection 6.1 cardiac perfusion, harvested slice 6.2 laser confocal microscopy CFSE-labeled MSCs in vivo survival and the secretion of growth factors VEGF and bFGF. the 6.3 reverse transcription - polymerase chain reaction (Reverse Transcription Polymerase ChainReaction, and RT-PCR) assay of VEGF and bFGFmRNA expression changes. 6.4 Immunohistochemical detection of factor VIII-related antigen expression 6.5 Basso-Beattie-Bresnahan (BBB) ??score and climb the mesh training assess rat behavior changes of 7. statistical analysis : 1. confocal laser microscope via the tail vein transplantation of MSCs can reach the spinal cord injury at the gathering after spinal cord injury, survival, and can secrete growth factors VEGF and bFGF. 2.RT-PCR results show the VEGF and bFGFmRNA expression after injury than normal increased; 1 day after transplantation, the MSCs transplantation group compared with the control group (P gt; 0.05) was not statistically significant; 3 days and 14 days after transplantation, the MSCs transplantation group were higher than the control group of the same time period and over time extension of expression continues to rise. 3. Factor VIII-related antigen immunohistochemistry results: one day after the transplant, the MSCs transplantation group compared with the control group (P gt; 0.05), no statistically significant; 3 and 14 post-transplant days, MSCs transplantation group microvessel density (microvessel density, MVD) were higher than the control group of the same time period and expression continued to increase with time. 4.BBB score and climb the mesh training transplanted MSCs can promote spinal cord injury hindlimb motor functional recovery. conclusion: vein graft MSCs can survive in the spinal cord injury and secretion of growth factors VEGF and bFGF, thereby promoting angiogenesis.

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
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