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Schwann cells in the peripheral nervous system axon damage repair plays a vital role. With the development of tissue engineering technology, some scholars have proposed using Schwann cells to repair damage to the central nervous system. However, due to Schwann cells terminally cells, their sources and limited proliferative capacity in vitro more difficult, it is difficult to obtain a sufficient number of cells, and can not fully meet the requirements and characteristics of seed cells. MSCs were found to be the source of seed cells provides a new way of thinking, research shows, BMSCs in vivo can differentiate into Schwann cell class. ADSCs and BMSCs not only has very similar biological properties, and in the expression of cell surface markers is also very similar. Compared with BMSCs, ADSCs have a wide source of easily obtained, rich content, easily cultured in vitro proliferation, etc., so that it is more and more people pay attention, and is likely to replace BMSCs, become a new source of seed cells for tissue engineering . In this experiment, SD rats inguinal fat extraction and training, access to fat stem cells in vitro and induced to differentiate into Schwann cells. Then ADSCs source Schwann cell transplantation, treatment of sports injury in rat cortical hemiplegia model, and achieved initial success. Methods: Using enzyme digestion, SD rats inguinal adipose tissue, to add a 10? S of sugar DMEM medium in 5% CO2, saturated humidity, 37 ℃ incubator cultured and passaged. 4th generation Asian fusion using state cell culture solution, drawing on BMSCs to differentiate into Schwann cells oriented approach to β-ME, ATRA, Forskolin, Heregulin, bFGF, BDNF and other sequential certain way under the effect of in vitro differentiation . Parallel immunohistochemistry Schwann cell-specific markers GFAP, S-100. While under the microscope lesioned rats motor cortex to produce a rat model of hemiplegia. Finally, adipose stem cells derived Schwann cells transplanted into damaged rat cerebral cortex partial paralysis, observational studies in rats recovery of motor function and using a more objective evaluation longo rated the degree of recovery of motor function. SPSS13.0 software for statistical analysis of all the data rows. Results: The success of the use of type I collagenase from SD rats inguinal adipose tissue isolated ADSCs, cultured in vitro proliferation and passage, and the use of bone marrow stem cells induced to differentiate into Schwann cells, also induced differentiation of Schwann cells in class , the performance of Schwann cell morphology, immunocytochemistry staining to the large number of cells expressing Schwann cell-specific markers GFAP, S100. Finally ADSCs source Schwann cells transplanted into the motor cortex brain injury rat model hemiplegia damaged part, compared with the control group, significant improvement in motor function, there are statistically significant. Conclusions: 1. Using collagenase type I can from SD rats inguinal adipose tissue isolated and cultured ADSCs, ADSCs can containing 10% FBS and 2mmol / L of L-glutamine sugar DMEM culture medium proliferation. 2.ADSCs can be induced to differentiate in vitro into classes Schwann cells, and express their specific markers. 3.ADSCs derived Schwann cell transplantation significantly improved motor cortex injury in rat model of postoperative hemiplegia motor function.
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