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Involvement of Cathepsin K in the Pathomechanism of Human Intervertebral Disc Degeneration

Author: WangZongLiang
Tutor: LiuWeiGang
School: Shihezi University
Course: Surgery
Keywords: Cathepsin K Disc Degeneration Matrix
CLC: R681.53
Type: Master's thesis
Year: 2008
Downloads: 49
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Abstract


Objective: This study by contrast detection of cathepsin K (Cathepsin K, Cath-K) expression in normal and degenerative disc further explore the Cath-K relations in the human intervertebral disc extracellular matrix changes in physiological and pathological conditions, and explore the Cath-K and disc degeneration, provide a theoretical basis for the mechanism of disc degeneration and delay treatment of intervertebral disc degeneration. Methods: 30 patients with lumbar disc herniation of intervertebral disc tissue in the Cath-K by immunohistochemical SP method and ELISA testing, and to take spinal fracture surgery patient specimens seven cases and fresh autopsy specimens of 8 normal intervertebral disc tissue of 15 patients for control. Experiment with intervertebral disc specimens were obtained from surgery (March 2006 to April 2007 in Xinjiang Shihezi University School of Medicine bone section of spinal surgical treatment of patients) and fresh corpses. The degeneration group from the lumbar disc herniation line MED surgery patients with normal group, taken from the disc herniation, spinal fractures in patients with voluntary contributions and autopsy disc specimens, specimens were taken in the intraoperative MRI T2-weighted signal changes and postoperative pathological examination , autopsy specimens by pathological examination verify the reliability of the specimens. Each specimens routine HE staining pathological diagnosis will be made of the the 4μm biopsy specimens by immunohistochemical streptavidin biotin-peroxidase method (S ─ P method) the slices were anti-Cath-K monoclonal antibody staining, observed under the microscope color, randomly selected from five high-power field, Cath-K in the cytoplasm stained brown positive, counting the percentage of positive cells and observed staining intensity, both product and score as a positive strength judgment based on positive intensity judgment, parallel line positive rate statistics statistical analysis. ELISA method for the determination of Cath-K line homogenates for statistical analysis. Results: Immunohistochemical staining Cath-K was localized in the cytoplasm. In the experimental group, the majority specimens Cath-K-positive cells more positive rate gt; 90%; cath-K fewer positive cells in the control group, the positive rate lt; 20%. Experimental group Cath-K protein expression was significantly higher than that in the control group, a statistically significant (P lt; 0.001), the ELISA method experimental data Cath-K content was 298.77 ± 105.97 pmol / ml, the control group in the Cath-K content of 182.24 ± 91.89 pmol / ml, statistics show that the experimental group was significantly higher than that in the control group, significant difference (P lt; 0.001). Conclusion: Cath-K is involved in the pathological changes of intervertebral disc degeneration, disc degeneration there is a correlation.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Bone diseases > Spine and back disorders > Spinal joint disease
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