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1. Research on Blood-Brain Barrier in Tracranical Tumors of Children 2. Application of Histochemistry in the Diagnosis and Differential Diagnosis of Hirschsprung Disease

Author: ZhuJin
Tutor: JinXianQing
School: Chongqing Medical University
Course: Pediatrics
Keywords: Multidrug resistance Blood-brain barrier Tumor Megacolon Histochemical Immunohistochemistry Diagnosis
CLC: R726.5
Type: Master's thesis
Year: 2008
Downloads: 51
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Abstract


The purpose of the first part of the children with intracranial tumor blood-brain barrier mechanism: by detecting multidrug resistance gene protein expression in children with intracranial tumors and normal brain tissue, explore the intracranial tumor resistance mechanisms. Methods: Immunohistochemistry was used to detect 30 cases of children with intracranial tumors and normal brain tissue in the P-glycoprotein (P-glycoprotein, P-gp) and multidrug resistance-associated protein (Multidrug resistance-associated protein, MRP) lung resistance-associated protein (Lung resistance-related protein, LRP), DNA topoisomerase II (Topoisomerase II, TopoII) and glutathione-S-transferase (with Glutathione-S-transferase-π, GST-π ) five kinds of multidrug resistance gene expression of the intensity and distribution. Results: P-gp is mainly expressed in the capillary endothelial cells, glial cells, the final foot and peritubular tissue, lower expression in tumor cells. The other four kinds of multi-drug resistance protein expression. Capillary wall Conclusion: P-gp in the blood-brain barrier in the capillary endothelial cells and glial cells enough to participate in the final block of chemotherapy drugs, increased intracranial tumor resistance. TopoII also involved in the blood-brain barrier formation mechanism. The second part of the purpose of histochemistry in the diagnosis and differential diagnosis of Hirschsprung: cholinesterase staining and antibody immunohistochemical examination, histochemical congenital megacolon (Hirschsprung disease, HD) class edge disease (Hirschsprung's allied disease, HAD) in the diagnosis and differential diagnosis. : Select osmium of Black Law cholinesterase staining, a rapid method cholinesterase staining and Bcl-2, calcium retinal protein (Calretinin, CR), neuron-specific enolase (Neuron Specific Endolase, NSE), S -100 protein (S-100) and cathepsin D (Cathepsin D, CAD), respectively after staining HD expansion segment, transitional segment and spasm segment intestinal wall specimens. Results: osmium black staining better, and fast method is less time-consuming. Bcl-2 for the degeneration or immature ganglion cells positive expression, no expression of nerve fibers in the ganglion cell degeneration bowel CR. NSE, S-100 and CAD are complementary to the observation of the plexus. Conclusion: The the osmium black for HD preoperative diagnosis, rapid method is more suitable for rapid diagnostic surgery. Bcl-2, CR-specific expression in the Diagnosis of HAD and neonatal HD can play an important role.

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CLC: > Medicine, health > Pediatrics > Pediatric surgery > Surgery in children of all physiological systems
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