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Objective: modern humans and experimental animal studies rekindled interest in the study of the relationship between uric acid and vascular function. Uric acid has anti-oxidative stress role as an antioxidant, is a protective factor, but more studies have shown that the blood uric acid for vascular function unfavorable occurrence and development with a variety of vascular diseases have correlation. Myeloperoxidase (MPO) is secreted by activated neutrophils the peroxidase, the various factors lead to vascular injury, neutrophil activation, thereby increased secretion MPO. MPO enhanced the role of oxidative stress can aggravate the injury of vascular endothelial function, promote atherosclerosis, is one of the indicators of oxidative stress and endothelial injury. Endothelin (ET) secretion and release of vascular endothelial cell polypeptide 21, has been confirmed in ischemia, hypoxia and ischemia-reperfusion injury in many pathological processes of vascular endothelial cell injury, is of great significance, endothelial cell-dependent the stable expression of ET for maintaining vessel base tension essential. Endothelial cell dysfunction disease, such as hypertension, heart failure, arterial atherosclerosis ET increased or increased activity. Endothelial dysfunction is an early manifestation of atherosclerosis. Artery ultrasound measurements of carotid intima-media thickness (IMT) is a commonly used indicator of the degree of atherosclerosis in clinical testing. The majority of the studies focus on the association between high levels of uric acid and atherosclerosis is not yet concerned about the low level of serum uric acid and normal levels of serum uric acid and vascular endothelial function and atherosclerosis associated research. This study conducted a cross-sectional study, correction factors that affect vascular function, detection the different blood uric acid level of MPO, ET, and IMT values ??change, different blood levels of uric acid on vascular function and atherosclerosis risk populations - patients with metabolic syndrome artery atherosclerosis. Methods: by 2005, the International Diabetes Federation (IDF) metabolic syndrome diagnostic criteria, except for blood diseases, primary liver and kidney disease, gout, malignant tumors and thyroid disease, 90 patients selected for metabolic syndrome. All subjects caught collecting blood pressure, body mass index, smoking history, family history and other information. And measuring fasting venous blood uric acid, blood glucose, blood lipids, liver and kidney function. Using colorimetric determination of MPO, measured by radioimmunoassay ET Doppler ultrasonic testing IMT. Uric acid levels were divided into three groups: A group (low blood uric acid group): uric acid lt; 176μmol / L. A total of 30 cases, including 16 male and 14 female, age 46 ~ 75 (63.80 ± 10.25) years old; B group (normal blood uric acid group): blood uric acid for men 176 to 416μmol / L, female 176 ~ 360 μmol / L, respectively. A total of 30 cases, including 17 males and 13 females, aged 44 ~ 79 (61.47 ± 12.23) years old; C group (high blood uric acid group): blood uric acid for men gt; 416μmol / L, female gt; 360 μmol / L,. A total of 30 cases, including 17 males and 13 females, aged 42 ~ 74 (58.90 ± 12.27) years old. Results: After adjustment for possible influencing factors, comparing three groups of subjects MPO, ET, IMT results show: the show through the results of the analysis of variance comparison: from Group A to Group C MPO level gradually increased. Group C MPO levels in patients with the highest (101.93 ± 10.25μg / L), followed by group B (91.38 ± 15.32μg / L), A set of minimum (the 42.73 ± 10.25μg / L). Group C and Group B patients with MPO were significantly higher than group A and group B and group C also has a statistically significant difference (p lt; 0.05) a statistically significant difference (p lt; 0.05). (2) by analysis of variance comparing results: from Group A to Group C, ET levels gradually increased. Hyperuricemia group (C) in patients with the highest level of ET (143.95 ± 57.90Pg/ml), followed by group B (84.90 ± 16.87Pg/ml), A group was the lowest (80.31 ± 29.55Pg/ml). ET levels in patients in group C was significantly higher than that in group A and group B (p lt; 0.05). Was no statistically significant difference between group A and group B (p gt; 0.05). ③ by analysis of variance comparing results: gradually increased from group A to group C, IMT value. Hyperuricemia group (C) in patients with the highest IMT values ??(1.07 ± 0.30m / s), followed by group B (0.88 ± 0.40m / s), group A, the lowest (0.81 ± 0.23m / s). IMT values ??of group C patients was significantly higher than that in group A and group B (p lt; 0.01). Was no statistically significant difference between group A and group B (p gt; 0.05). ④ three groups of subjects, the highest incidence of C patients with carotid atherosclerosis was 63.3% (19/30), followed by group B, 30.0% (9/30), group A was 20.0% (6/30). The three sets of values ??for multiple rate comparison of the chi-square test results showed that the positive rate of the three groups of subjects statistically significant difference (p lt; 0.05). The incidence of group C patients with carotid atherosclerosis were significantly higher than that in group A and group B patients (p lt; 0.05). (5) different levels of uric acid as independent variables, respectively, as the dependent variable MPO, IMT, ET, Pearson correlation analysis, the results show: the level of uric acid and MPO was positive correlation, the correlation coefficient = 0.787, statistically significant correlation ( p = 0.000); levels of uric acid in the ET value was positive correlation, correlation coefficient = 0.301, statistically significant (p = 0.004); uric acid levels were also positively correlated with IMT level, correlation coefficient = 0.541, correlation was statistically significance (p = 0.000); MPO level was positively correlated with IMT values, the correlation coefficient = 0.241, correlation was statistically significant (p = 0.022). ⑥ using stepwise regression analysis model to analyze the impact factors of the metabolic syndrome in patients with MPO levels. MPO as the dependent variable and age, BMI, systolic blood pressure, FPG, LDL-C, HDL-C, TG, TC, NO, IMT and ET may for the factors affecting MPO values ??as independent variables to the regression analysis, the results show : independent prognostic factors of the metabolic syndrome in patients with MPO uric acid levels (p lt; 0.05). ⑦ stepwise multiple regression analysis model to analyze the impact factors of the metabolic syndrome ET level. ET as the dependent variable and age, BMI, systolic blood pressure, FPG, LDL-C, HDL-C, TG, TC, NO, IMT and MPO and other factors may affect the level of ET as the independent variable regression analysis, the results show : ET levels in patients with metabolic syndrome independent prognostic factors including uric acid levels and HDL-C (p lt; 0.05). ⑧ stepwise multiple regression analysis model to analyze the factors that affect metabolic syndrome IMT values. The IMT as the dependent variable and age, BMI, systolic blood pressure, FPG, LDL-C, HDL-C, TG, TC, NO, ET and MPO may for the factors affecting IMT value as independent variables to the regression analysis, the results show : The metabolic syndrome IMT values ??independent factors including uric acid levels and HDL-C (p lt; 0.05). Conclusion: (1) patients with metabolic syndrome, with increased serum uric acid levels from low blood uric acid level to high blood levels of uric acid, blood MPO levels and ET have gradually increased the trend, and serum uric acid levels were also affecting the blood MPO level and the level of ET independent factors. Tip patients with metabolic syndrome, increased blood uric acid level risk factors that affect vascular endothelial function. (2) metabolic syndrome from low blood levels of uric acid to high blood levels of uric acid, IMT values ??increased gradually with increased blood uric acid level, and the number of patients with carotid atherosclerosis also gradually increased, blood uric acid levels affect IMT The value of the independent factors. Tip patients with metabolic syndrome, blood uric acid levels affect the extent of atherosclerosis risk factors.
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