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Berberine anti-human highly metastatic lung cancer cells induced human umbilical vein endothelial cell apoptosis research

Author: HePing
Tutor: HuangQiFu；WangYi
School: Beijing University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Apoptosis Berberine Ca2+ Laser Scanning Confocal Microscope pH Tumor metastasis
CLC: R734.2
Type: Master's thesis
Year: 2008
Downloads: 101
Quote: 0
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Abstract


Tumor metastasis is the primary cause of failures in antineoplaston and death of patients;therefore,how to control tumor metastasis is the key to decide the prognosis of cancer patients.The tumor metastasis is a complex and multi-step process.Hematogenous metastasis is the most important way.The interaction of tumor cell and the vascular endothelial cell is the most complex and the most important link in hematogenous metastasis.Some research indicate that tumor metastasis is followed with vascular endothelial cell’s shrinkage and apoptosis, the second messenger Ca2+density and the pH change in the vascular endothelial cell have a close correlation with apoptosis.The berberine is a common medication in the digestive system’s infection.Some researches indicate that berberine also has the function to restrain the cancer cell’s growth in resent years.We have also conducted preliminary study in this function,discovering that the berberine has the function to restrain the lung cancer cell’s multiplication,the growth of solid tumor and to reduce lung-metastasis. But its mechanism in anti-tumor metastasis also needs further research.The Laser Scanning Confocal Microscope（LSCM）is a important method in the modern biology used in the non-damage observation of living cell and organization.Combined with specificity fluorescent probe,it widely applies in monitoring some important biochemical ingredient in spatial distribution and change along with the time in the living cell level,thus to obtain the information of the vital movement process.This experiment will use LSCM to research the protective function that berberine on the PG cell to cause the apoptosis of human umbilical vein endothelial cell（HUVEC）,expounds the mechanism to resist tumor metastasis.1 GoalTo research the protective function which berberine to PG cell causes HUVEC apoptosis,expounded the mechanism that berberine resist tumor metastasis.Using LSCM to observe influence that berberine on the tumor metastasis,to expound the mechanism of action between drug and cell,and open a new research way for the traditional Chinese medicine research. 2 MethodFirst we use the MTT method to screen the most appropriate density and the time which function between the HUVEC and PG cell to culture supernatant,with the PG cell to culture supernatant and function with the HUVEC,establishing the tumor attacking model.The medicine protection group is using the berberine to process HUVEC before the PG cell culture supernatant function with the HUVEC.We use the MTT method to detect the cell’s survival percentage in different medicine density and different medicine response time,to determine that the best medicine dosage and the best response time with the berberine.Then we carries on the observation and analysis aiming directly at the HUVEC percentage of apoptosis,the shape of nucleolus,the Ca2+ density and the pH value change and so.3 Result（1）Berberine can obviously reduce the apoptosis percentage of HUVEC attacked by tumor.（2）Berberine can relieve the deformation of nucleolus,the concentration of chromatin and so on.（3）Berberine can relieve the calcium overload of HUVEC.（4）Berberine can restrain the depression of pH value.4 ConclusionThe berberine can obviously reduce the HUVEC damage caused by tumor,making the cell structure and the function restore normally.One of these kinds of possibly protective mechanisms is to restrain apoptosis through reducing the excessive load of the Ca2+in the cell and the pH reducing.

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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