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Research purposes and the background: reactive oxygen species (ROS) are the major free radicals, of the living body is a group containing compound of the oxygen-containing functional groups of the chemically active. It includes super oxides, nitrogen oxides, hydroxyl groups, singlet oxygen and other molecular structures, such as hydrogen peroxide (H 2 O 2 ), over oxidation of nitrite, the majority of cells have a toxic effect. Under normal circumstances, the generation of ROS and removals of the machine body in a state of dynamic equilibrium. When the body is in the state of oxidative stress in vivo tissue the ROS amount will rise, scavenging over the body, resulting in tissue lipid peroxidation levels, causing oxidative DNA damage and protein expression abnormalities, cause damage to the body. It is in many acute myocardial diseases, such as myocardial ischemia-reperfusion injury, heart failure, and plays an important role in the occurrence and development of hypertension. H 2 O 2 as a form of reactive oxygen species, has a strong lead to the role of lipid peroxidation, almost can react with any cellular components, causing the chain lipid peroxidation oxidation reaction, resulting in myocardial cell apoptosis and dysfunction. The curcumin (curcumine) is extracted from the rhizomes of Zingiberaceae Turmeric (Curcuma longa L.), a phenolic pigment composition, color stability and low toxicity, has been widely used in food additives and dyes. Many research results suggest that curcumin is to protect normal cells from a variety of adverse factors, injury to play its anti-inflammatory, anti-atherosclerotic, anti-tumor, anti-oxidation, a series of pharmacological effects, is currently the most compelling attention also get more experimental proof of its radical scavenging effect. Curcumin as an oxygen radical scavenger, significantly inhibiting active oxygen such as superoxide anion, hydrogen peroxide, nitrous acid salt group generated. Curcumin on hydrogen peroxide-induced fibroblast and endothelial cell injury can play a strong antagonistic effect. Mechanism may mitigate curcumin can isoproterenol-induced rat ischemic electrocardiographic changes to improve the capacity of rat myocardial hypoxia tolerance, has a protective effect on myocardial ischemic injury, speculated that the protective effect its free radical scavenging. But so far, curcumin in vitro cellular level, the role and mechanisms of myocardial ischemic injury has not been confirmed. Of this study is to by neonatal rat cardiomyocytes cultured in vitro, to establish hydrogen peroxide myocardial injury model, effects of curcumin on the role too neonatal rat cardiac cells from oxidative damage induced by hydrogen peroxide, which provide the experimental basis for curcumin prevention and treatment of cardiovascular disease. Research methods: (1) primary cultured neonatal rat cardiomyocytes were randomly divided into blank control group, the control group of oxidative damage and different concentrations of curcumin group; (2) using the MTT assay (D MTT Trace enzyme reaction colorimetry) Determination of myocardial cell activity; (3) using the kit myocardial cells of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD) activity was measured; (4) flow cytometry to detect apoptosis. Results: (1) compared with the normal group myocardial cells, H 2 O 2 group myocardial cell viability decreased its OD value was significantly lower than that in the normal control group; addition to curcumin group of low-dose, high-dose curcumin group significantly improved H 2 O 2 myocardial cell damage caused by myocardial survival, and with turmeric increase of the concentration of the hormone, the OD value of the myocardial cells is increased gradually. (2) H O 2 group SOD activity was significantly lower than that in normal control group was significantly higher than the normal control group, while MDA and LDH; All dose curcumin group could reduce the H 2 O 2 cardiomyocytes injury LDH and MDA production, improve the activity of SOD, the changes are more obvious with increasing doses of curcumin. (3) H 2 O 2 injury group cardiomyocyte apoptosis rate was significantly higher than the normal control group, while the H 2 O 2 and 50μmol / L curcumin role group, the apoptosis rate than H 2 O 2 injury group decreased significantly. CONCLUSION: Curcumin H 2 O 2 damage myocardial cells have a protective effect, and dose-dependent manner, and its mechanism may scavenge oxygen free radicals and curcumin.
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