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The native copper (Pyritum) is a commonly used Chinese herb, in traditional bone prescriptions account for a large proportion, Oral topical Safe, plays a decisive role in traditional Chinese medicine. But the natural copper is often rough to the undissolved state mineral raw powder medicine, resulting in a large amount of insoluble active ingredients. Thus the native copper and native copper containing proprietary Chinese medicines in the pharmaceutical market, has been limited. In this paper, our laboratory isolation and identification of a plant height of activity acidophilic Thiobacillus ferrooxidans (Acidithiobacillusferrooxidans, At.f) for processing natural copper mineral drugs, preliminary inspection of the impact of natural copper leaching solution of osteoblast proliferation, differentiation and promote fracture healing and prevention of osteoporosis efficacy experiments carried out a preliminary study of the natural copper leaching solution. The natural copper leaching solution of iron, copper, manganese, zinc, selenium metal ion concentration determination, the results acidophilic Thiobacillus thiooxidans of the native copper leaching role. 15 days after the solution of soluble iron concentration 1592μg/mL, the leaching rate of 68.33%. The concentration of other metals also increased compared with before leaching. MTT assay nitrophenylphosphate method to observe the different concentrations of native copper leaching solution on cultured osteoblast proliferation, alkaline phosphatase activity. The results show that: the natural copper leaching solution at a concentration of 1/40, 1/80 two, significantly inhibited the proliferation of osteoblasts, and other concentrations no effect on cell proliferation. Leaching solution of ALP activity in a concentration of 1/2560, having a significantly enhanced. Explore the natural copper leaching solution dexamethasone induced osteoporosis prevention role. SD rats were randomly assigned to administration, six weeks after the determination of the concentration of serum calcium, alkaline phosphatase activity and femoral bone mineral density, bone mineral content and femoral histological slices observed. The results showed that: no change in the concentration of serum calcium, serum alkaline phosphatase activity of the experimental group was significantly higher than the negative group. Rat femur bone mineral density and bone mineral content did not change. Femoral histological observation found that the femoral trabecular structure of the experimental group compared with the negative group arranged closely, so preliminary evidence of natural copper leaching solution of a certain role in the prevention of osteoporosis, further research is needed. Explore the role of natural copper leaching solution of rat fracture healing. SD rats were randomly divided into three groups, positive group, the experimental group, negative group, respectively, the concentration of serum calcium, alkaline phosphatase activity was measured at 14, 21 days, and observation of X-rays, histological sections. The results show that: the calcium concentration in 14 and 21 days of the experimental group were lower than the negative group, and the difference is significant. In 14 days, the serum ALP activity of the experimental group were higher than the negative group, and the difference is significant. X-rays showed that, in 14 and 21 days, the experimental group, the fracture callus area greater than the negative group. Histological sections showed that, in 21 days, the experimental group cartilage bone callus and trabecular bone formation and bone healing aspects significantly earlier than the negative group.
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