|
Kiwi roots high medicinal value, civil primarily for the treatment of hepatitis, gastrointestinal tumor embolism. Modern Pharmacy roots of kiwifruit research has focused on chemical composition and antitumor activity analysis, and on the kiwi roots anti-virus, anti-tumor active site of Screening was not reported, limiting the further development of the herbs. Based on this paper carried out research in this area. [Objective] The main subject of medical experiments through screening Kiwi roots anti-tumor, anti-viral activity sites, initially identified the approximate composition of its active site category, for the further development Kiwi roots foundation. [Method] 1, with heating reflux, column chromatography and thin layer chromatography extraction and separation of kiwi root; 2, select the Bel-7402, SW-620, MDA-MB-231 three kinds of tumor cell lines by MTT assay, growth curve method and apoptosis staining experiments screening Kiwi roots anti-tumor site; 3, using the 2.2.15 cell model by MTT assay and HBsAg, HBeAg detection, screening anti-hepatitis B virus active site. [Results] antitumor screening results showed that the active site of ethyl acetate extract part (Y 1 Y 2 ), ethanol extraction section (A 1 A 3 A 4 , A 1 A 4 A 6 ), the mixing section ( A 1 A 4 Y 1 ) for the three kinds of tumor cells were strongly inhibited, and the ethanol extract of kiwifruit root portion (A 1 A 4 A 6 ), the mixing section (A 1 A 4 Y 1 ) can induce apoptosis. Determination Show: A 1 A 4 A 6 content of 54.35%, A 1 A 4 Y 1 content of 62.64%. Kiwi roots anti-hepatitis B virus screening results showed that the active site: ethanol extraction section (A 1 A 3 A 4 ), extracted with ethyl acetate portion (Y 1 Y 2 ) on 2.2.15 cells secrete HBeAg, HBsAg of TI gt; 2, tips for both inhibition. According assay results ethanol extract part (A 1 A 3 A 4 ) content of 60.28%. The foam experiments, acetic anhydride preliminary identification of concentrated sulfuric acid anti-tumor and anti-viral activity of the chemical constituents of triterpenoids. [Conclusion] According to the results of the preliminary view A 1 A 4 A 6 , A 1 A 4 Y 1 is the root of the anti-tumor activity of kiwifruit site, A 1 A 3 A 4 is Kiwi roots anti-hepatitis virus active site. In vitro there is a strong anti-tumor and anti-hepatitis effect, its mechanism may be related to induction of apoptosis. Little effect on normal cells. To further separation of anti-tumor, anti-hepatitis ingredients foundation.
|