Dissertation > Excellent graduate degree dissertation topics show

The Effects of Yi-Qi Huo-Xue Chinese Herbs on Expressions of TSP-1、TSP-2 and Their Receptor CD36 Protein in Intracerebral Hemorrhagic Rat Brains

Author: ChenBaiLin
Tutor: XingZhiHua
School: Central South University
Course: Chinese and Western Medicine
Keywords: Cerebral hemorrhage Yiqihuoxue medicine Thrombospondin -1 Thrombospondin -2 CD36 receptor Western blot
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 79
Quote: 0
Read: Download Dissertation


Purpose and Background: cerebral hemorrhage (intracerebral hemorrhage, ICH) accounted for about 35% of cerebrovascular disease, have a high morbidity and mortality, 30-day mortality was 50%, 40% of survivors were left severely disabled, Only 10% of patients after the disease can live independently, is a threat to the health of the elderly is one of the major diseases. Cerebral hemorrhage are Chinese, \20 Since the 1980s, many scholars, according microcosmic syndrome and Chinese medicine theory \pathogenesis, blood circulation is the key to treatment. Wang Ren under which \absorption and brain edema, inhibiting the inflammatory response and so on. Damage zone with hematoma cerebral hemorrhage, edema absorption, inflammation subsided, the destruction of the microvascular system necessarily through angiogenesis in the lesion area reconstruction to restore the damaged area of ??the blood perfusion for brain tissue repair foundation. Angiogenesis by inhibiting angiogenesis factor and dynamic balance adjustment factor to complete. Thrombospondin -1 (thrombospondins-1, TSP-1) and thrombin-sensitive protein -2 (thrombospondins-2, TSP-2) of the TSR sequence binds to its receptor CD36, can inhibit endothelial cell migration, endothelial cells, thereby inhibiting angiogenesis, inhibition of angiogenesis is an important factor. Therefore, we speculate that there may Yiqihuoxue medicine after cerebral hemorrhage by affecting the TSP-1, TSP-2 and its receptor CD36 protein expression, adjusting the body's inhibition of angiogenesis, vascular reconstruction process change, and promote brain tissue repair, and play its \In this study, immunoblotting (western-blotting) detecting cerebral hemorrhage rat brain TSP-1, TSP-2 and CD36 expression by observing the TSP-1, TSP-2 and CD36 protein expression in cerebral hemorrhage damage zone rules and after the Chinese intervention Yiqihuoxue variation to preliminary study Yiqihuoxue bleeding in the brain damage zone in the mechanism of revascularization. Methods: 155 SD rats were randomly divided into normal group, sham operation group, model group, Yiqihuoxue group, Qi and blood group group, stereotactic injection to the brain type Ⅶ collagenase copy globus pallidus ICH model the corresponding group were fed BYHWD and the party's qi and blood drug group drug group. Modeling and in each group after administration 1d, 4d, 7d, 14d, 21d and 28d (normal rats at 1d) were randomly selected five rats, decapitation obtain cerebral hematoma brain tissue by Western blotting using normal group and other groups at each time point TSP-1, TSP-2 and CD36 expression. Experimental data were analyzed by SPSS12.0 statistical software. Result: the normal group and sham operation group at different time points TSP-1, TSP-2 and CD36 protein expression did not change. Model group, TSP-1 protein expression after cerebral hemorrhage 1d beginning, 4d peak, 4d-14d gradually decreased, 14d-28d at each time point expressed indifference. Qi and blood group and blood group TSP-1 protein expression in 1d and 4d are lower than the model group (P <0.01), Qi group TSP-1 protein expression in 4d than the model group (P <0.01): the model group TSP -2 protein expression after cerebral hemorrhage began 1d, 1d-7d each time point showed no significant difference, 7d-28d expression showed a gradual upward trend and reached a peak in the 28d. Yiqihuoxue group TSP-2 protein expression in 14d-21d rise slower than the other three groups (P <0.01), the expression in the 21d weaker than the other three groups (P <0.01), the expression in the 28d stronger than the other three groups (P <0.01); model group CD36 protein expression after cerebral hemorrhage began 1d, 4d peak, 4d-14d gradually decreased, 14d-28d to 28d again began to rise and reached a peak, 4d CD36 protein expression in the model group than the other three groups ( P <0.01), 28d Yiqihuoxue group of CD36 protein expression than the other three groups (P <0.01) Conclusions: 1. intracerebral hemorrhage District TSP-1 protein expression in the early strong, TSP-2 protein expression in the late strong bimodal CD36 protein expression. 2 Yiqihuoxue medicine is possible by changing the TSP-1, TSP-2 and CD36 protein levels, dynamic regulation of angiogenesis inhibition mechanisms to promote microvascular reconstruction and cerebral tissue repair.

Related Dissertations

  1. A Control Study about Small Bone Window Hematoma with Hematoma of Endoscopic in the Basal Ganglia Intracerebral Hemorrhage,R651.11
  2. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34
  3. The Significance and the Dynamic Changes of Peripheral Blood Cd34+、 Cd166+ and the White Blood Cells after Hypertensive Intracerebral Hemorrhage,R743.34
  4. Index Variation of Clinical Indicators in Patients with Intracerebral Hemorrhage (ICH) and Its Association with 15 STR Loci,R743.34
  5. The Regulation and Significance of NHE1 in Acidic Micro-environment of Gastric Carcinoma,R735.2
  6. Integrative treatment of acute hypertensive cerebral hemorrhage,R743.3
  7. Study on the Effect and Mechanism of Tamoxifen on the Radiosensitivity in Glioma Cell SHG44,R737.9
  8. Effects of 60Coγ Radiation Exposure on Expressions of CDKN1A, FABP4 and HSL in Human Lymphocytoblast,R144
  9. The Expression Research of PMP22 Gene in Bombyx Mori,Q78
  10. The Effect of Different Concentration of Urokinase on Stereotactic Aspiration of the Rabbit Intracerebral Hematoma and the Expression of the Heme Oxygenase-1,R743.34
  11. The Changes of Serum Thrombospondin-1 and Plasminogen Activator Inhibitor-1 in Patients with Congenital Heart Disease Complicated with Pulmonary Hypertension,R543.2
  12. Atorvastatin treatment of hypertensive intracerebral hemorrhage in rats Experimental study of mechanism of action,R965
  13. The Relation between Expression of IL-8、 ICAM-1 in Tissue Surrounding the Hematoma and Brain Edema in Experimental Intracerebral Hemorrhage and the Intervention Role of Injection of Ginkgo Biloba Leave Extract,R743.34
  14. Expression of ADAMTS-2 and TGF-β1 in Liver Cirrhosis,R575.2
  15. Identification of Ts21 Recombinant Protein of Trichinella Spiralis and Its Diagnostic and Preventive Value,R446.6
  16. Identification, Expression and Functional Characterization of Properdin from Zebrafish,Q75
  17. Molecular Cloning, Expression and Antimicrobial Resistance of Transferrin from Asian Yellow Pond Turtle,S917.4
  18. The Clinical Significance of Von Willebrand Factor Cleaving Protease (ADAMTS13) and Thrombospondin1 in Acute Ischemic Stroke,R743.33
  19. The Changes of TSP-1, PDGF-B and the Intervention Role of TA in Early Diabetic Rats Retina,R774.1
  20. Correlation Research on the Expression of TSP-Ⅰ, IGF-Ⅱ, Contents of E2 and Pathogenesis of Hysteromyoma,R737.33
  21. Construction and Expression of Recombinant Extracellular Domain of Human ICOS in Escherichia Coli,Q78

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile