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The Effects of Yi-Qi Huo-Xue Chinese Herbs on Expressions of TSP-1、TSP-2 and Their Receptor CD36 Protein in Intracerebral Hemorrhagic Rat Brains

Author: ChenBaiLin
Tutor: XingZhiHua
School: Central South University
Course: Chinese and Western Medicine
Keywords: Cerebral hemorrhage Yiqihuoxue medicine Thrombospondin -1 Thrombospondin -2 CD36 receptor Western blot
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 79
Quote: 0
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Abstract


Purpose and Background: cerebral hemorrhage (intracerebral hemorrhage, ICH) accounted for about 35% of cerebrovascular disease, have a high morbidity and mortality, 30-day mortality was 50%, 40% of survivors were left severely disabled, Only 10% of patients after the disease can live independently, is a threat to the health of the elderly is one of the major diseases. Cerebral hemorrhage are Chinese, \20 Since the 1980s, many scholars, according microcosmic syndrome and Chinese medicine theory \pathogenesis, blood circulation is the key to treatment. Wang Ren under which \absorption and brain edema, inhibiting the inflammatory response and so on. Damage zone with hematoma cerebral hemorrhage, edema absorption, inflammation subsided, the destruction of the microvascular system necessarily through angiogenesis in the lesion area reconstruction to restore the damaged area of ??the blood perfusion for brain tissue repair foundation. Angiogenesis by inhibiting angiogenesis factor and dynamic balance adjustment factor to complete. Thrombospondin -1 (thrombospondins-1, TSP-1) and thrombin-sensitive protein -2 (thrombospondins-2, TSP-2) of the TSR sequence binds to its receptor CD36, can inhibit endothelial cell migration, endothelial cells, thereby inhibiting angiogenesis, inhibition of angiogenesis is an important factor. Therefore, we speculate that there may Yiqihuoxue medicine after cerebral hemorrhage by affecting the TSP-1, TSP-2 and its receptor CD36 protein expression, adjusting the body's inhibition of angiogenesis, vascular reconstruction process change, and promote brain tissue repair, and play its \In this study, immunoblotting (western-blotting) detecting cerebral hemorrhage rat brain TSP-1, TSP-2 and CD36 expression by observing the TSP-1, TSP-2 and CD36 protein expression in cerebral hemorrhage damage zone rules and after the Chinese intervention Yiqihuoxue variation to preliminary study Yiqihuoxue bleeding in the brain damage zone in the mechanism of revascularization. Methods: 155 SD rats were randomly divided into normal group, sham operation group, model group, Yiqihuoxue group, Qi and blood group group, stereotactic injection to the brain type Ⅶ collagenase copy globus pallidus ICH model the corresponding group were fed BYHWD and the party's qi and blood drug group drug group. Modeling and in each group after administration 1d, 4d, 7d, 14d, 21d and 28d (normal rats at 1d) were randomly selected five rats, decapitation obtain cerebral hematoma brain tissue by Western blotting using normal group and other groups at each time point TSP-1, TSP-2 and CD36 expression. Experimental data were analyzed by SPSS12.0 statistical software. Result: the normal group and sham operation group at different time points TSP-1, TSP-2 and CD36 protein expression did not change. Model group, TSP-1 protein expression after cerebral hemorrhage 1d beginning, 4d peak, 4d-14d gradually decreased, 14d-28d at each time point expressed indifference. Qi and blood group and blood group TSP-1 protein expression in 1d and 4d are lower than the model group (P <0.01), Qi group TSP-1 protein expression in 4d than the model group (P <0.01): the model group TSP -2 protein expression after cerebral hemorrhage began 1d, 1d-7d each time point showed no significant difference, 7d-28d expression showed a gradual upward trend and reached a peak in the 28d. Yiqihuoxue group TSP-2 protein expression in 14d-21d rise slower than the other three groups (P <0.01), the expression in the 21d weaker than the other three groups (P <0.01), the expression in the 28d stronger than the other three groups (P <0.01); model group CD36 protein expression after cerebral hemorrhage began 1d, 4d peak, 4d-14d gradually decreased, 14d-28d to 28d again began to rise and reached a peak, 4d CD36 protein expression in the model group than the other three groups ( P <0.01), 28d Yiqihuoxue group of CD36 protein expression than the other three groups (P <0.01) Conclusions: 1. intracerebral hemorrhage District TSP-1 protein expression in the early strong, TSP-2 protein expression in the late strong bimodal CD36 protein expression. 2 Yiqihuoxue medicine is possible by changing the TSP-1, TSP-2 and CD36 protein levels, dynamic regulation of angiogenesis inhibition mechanisms to promote microvascular reconstruction and cerebral tissue repair.

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