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Objective: A host plant is oleander safflower the Loranthaceae extract ( hereinafter referred to as Nispex) on cultured human Burkitt lymphoma cell line CA46 anti-tumor effect , and to explore the molecular mechanisms of its anti- tumor effect . Methods: The inhibitory effect the the MTT assay Nispex of CA46 cell proliferation , cell colony train the observation Nispex on in CA46 proliferation cell populations ; AO -EB fluorescent staining , in situ end labeling (TUNEL) assay, DNA gel electrophoresis analysis AnnexinV assay to detect apoptosis ; front and rear the RT - PCR detection Nispex of the role of bcl -2 , bax , c -myc gene mRNA levels , the CA46 cell after the the immunofluorescence assay Nispex role of NF-κB nuclear transfer changes , Western blot detection Nispex the CA-46 cells Ikk IκB the NF-κBp65 , Bcl -2 , Bax, caspase -3 and PARP, C- myc expression of apoptosis-related proteins affect . Results : Nispex significantly inhibit the proliferation of CA46 cells ; AO - EB staining method , the end of the in situ labeling (TUNEL) assay , DNA gel electrophoresis analysis and AnnexinV detection the law confirmed Nispex effective CA46 cells induced apoptosis ; RT-PCR detected CA46 cells after Nispex role bax gene increased mRNA expression of bcl- 2 , c - myc gene mRNA expression decreased immunofluorescence CA46 in the nucleus and cytoplasm of NF-κB the detected Nispex after significantly reducing Western blot detected Nispex CA46 cells after treatment Bax, Caspase-3 protein expression increased the Ikk the I- κB , Bcl -2 , the NF-κBp65 , PARP, C- myc protein expression levels decreased . Conclusion : Nispex can effectively inhibit the CA46 cell proliferation and induced apoptosis , and this effect may be related to the NF-κB signaling pathway .
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