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Tri pill was first seen in the \For blood stasis amenorrhea, menstrual pain. The basis of research by our group pre triangular pill for the treatment of endometriosis: SLW narrow the ectopic endometrium volume, triangular, Curcuma containing serum can inhibit the vascular endothelial growth factor (VEGF)-induced vascular endothelial cell proliferation. Endometriosis is an inflammatory disease, pain disorders, triangular pill has anti-inflammatory and analgesic effects is still unknown. The test triangular pill analgesic and anti-inflammatory effects, and to provide an experimental basis for the future development of new drugs to treat endometriosis. Purposes: observation triangular pill (SLW) analgesic and anti-inflammatory effects in animal models of pain and inflammation, and inflammation exudate prostaglandin E2 (PGE2) and histamine (Hist) and 5 - hydroxytryptamine (5-HT ) content, to provide for the preparation of pre-clinical studies pharmacodynamic data. Method: an analgesic effect test 1.1 hot-plate test screening pain reaction incubation period of 10 to 30 seconds Kunming female mice were randomly divided into five groups, respectively, were given triangular pill 1.43mg · kg -1 < / sup> · d -1 , 0.48mg · kg -1 · d -1 , 0.16mg · kg -1 < / sup> · d -1 , tramadol 20 mg · kg -1 · d -1 , the model group were given saline 0.2 mL · 10 g -1 · d -1 30min after the mice were placed on a hot plate of 55 ± 0.5 ℃. Determination of the mice in each group pain response latency. 1.2 writhing mice were randomly divided into 5 groups, respectively, were given a triangular pill 1.43mg · kg -1 · d -1 0.48mg · kg -1 · d -1 , 0.16mg · kg -1 · d -1 , celecoxib etoricoxib 25 mg · kg -1 · d -1 , the model group were given normal saline 0.2 mL · 10 g -1 · d < sup> -1 , daily gavage once continuous administration 3d, the 30min each mouse after the last administration intraperitoneal injection of freshly prepared 0.7% acetic acid solution of 0.1 mL · 10 g -1 . Writhing within 20 min of each group of mice was measured reaction times. 2 anti-inflammatory effects test 2.1 acetate abdominal capillary permeability increase in experimental mice, the animals were divided into six groups, were fed given Trigonobalanus pill 1.43mg · kg -1 · d -1 , 0.48mg · kg -1 · d -1 , 0.16mg · kg -1 · d -1 , plug to celecoxib 25 mg · kg -1 · d -1 , model group and the normal group were fed with physiological saline 0.2 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, 30 minutes after the last administration tail vein injection of 0.5% Evans blue solution of 0.1 mL · 10 g -1 , and immediate intraperitoneal injection preparation 0.7% acetic acid solution of 0.1 mL · 10 g -1 (normal mice intraperitoneal injection of acetic acid solution), the establishment of increased capillary permeability mouse model. Determination of the concentration of the solution of Evans blue in the abdominal cavity of the mice in each group. 2.2 ear edema mice were randomly divided into 5 groups, respectively, were given a triangular pill 1.43mg · kg -1 · d -1 0.48mg · kg -1 · d -1 , 0.16mg · kg -1 · d -1 , celecoxib etoricoxib 25 mg · kg -1 · d -1 , the model group were given saline 0.2 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, 30min each mouse were spread evenly right ear 100% xylene 0.03 mL / proinflammatory after the last administration, the establishment of inflammation in a mouse model. Each group of mice ear swelling was measured. 2.3 rat paw edema method to select male Wistar rats were randomly divided into five groups, were administered to give the triangular pill 1.02mg · kg -1 · d -1 , 0.34mg · kg -1 · d -1 , 0.11mg · kg -1 · d -1 , celecoxib 20 mg · kg -1 · d -1 , model group fed with normal saline 0.1 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, immediately after the last administration of each rat were right rear paw subcutaneous injection of 1% carrageenan 0.1 mL of / proinflammatory only establish a rat model of inflammation. Determination of each group rat paw edema. 2.4 tampon induced rat granulation tissue formation in experimental select male Wistar rats, cotton balls (each weighing 30 mg ± 0.5 mg) were implanted into rat on both sides of the groin subcutaneous establish a rat model of chronic inflammation. Successful modeling rats were divided into five groups, successful modeling 0d were administered given Trigonobalanus pill 1.02mg · kg -1 · d -1 0.34mg · kg -1 · d -1 , 0.11mg · kg -1 · d -1 , -1 · d celecoxib 20 mg · kg -1 , model group fed saline 0.1 mL of · 10 g -1 · d -1 , daily gavage once continuous administration 7d, 8d remove the cotton balls, Determination of the rats granuloma NW. 3 MECHANISM 3.1 rat carrageenan inflammatory exudate PGE2 in experiments male Wistar rats were randomly divided into 6 groups, respectively, were given triangular pill 1.02mg · kg -1 · d -1 , 0.34mg · kg -1 · d -1 , 0.11mg · kg -1 · d -1 , celecoxib 20 mg · kg -1 · d -1 , normal and model groups were fed with physiological saline 0.1 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, immediately after the last administration each rat right after subcutaneous injection of 1% carrageenan paw 0.1 mL / proinflammatory (normal group is not in the right rear paw subcutaneous injection of carrageenan), the establishment of a rat model of inflammation. Determination of rats inflammation enough PGE2 exudate amount. Hist content of 3.2 rat carrageenan inflammatory exudate experiments to select male SD rats were randomly divided into 6 groups, respectively, were given triangular pill 1.02mg · kg -1 · d -1 , 0.34mg · kg -1 · d -1 , 0.11mg · kg -1 · d -1 , celecoxib 20 mg · kg -1 · d -1 normal group and model group were fed with normal saline 0.1 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, at 30 minutes after the last administration of each rat right hind leg 0.1 mL / plantar subcutaneous injection of 1% carrageenan induced inflammation (normal group is not in the right rear paw subcutaneous injection of carrageenan), to establish a rat model of inflammation. Determination of the rats inflammation foot Hist oozing amount. 3.3 rat carrageenan vegetable gum inflammation exudate 5-HT content was selected SD male rats were randomly divided into 6 groups, respectively, were given triangular pill 1.02mg · kg -1 · d -1 , 0.34mg · kg -1 · d -1 , 0.11mg · kg -1 · d -1 , celecoxib 20 mg · kg -1 · d -1 , normal and model groups were fed with normal saline 0.1 mL · 10 g -1 · d -1 , daily gavage once continuous administration 3d, at 30 minutes after the last administration of each rat right The carrageenan paw after subcutaneous injection of 1% angle of 0.1 mL / proinflammatory (normal group is not in the right rear paw subcutaneous injection of carrageenan), establish a rat model of inflammation. Determination of rats inflammation of the foot 5-HT amount of seepage. 3.4 adrenalectomized mice ear swelling choose to adrenal mice were randomly divided into 5 groups, respectively, were given a triangular pill 1.43mg · kg -1 · d - 1 , 0.48mg · kg -1 · d -1 , 0.16mg · kg -1 · d - 1 , celecoxib etoricoxib 25 mg · kg -1 · d -1 , the model group were given normal saline 0.2 mL · 10 g -1 · d -1 , day gavage once continuous administration 3d, 30min each mouse after the last administration, the right auricle evenly coated 100% xylene 0.03 mL / proinflammatory only establish a mouse model of inflammation. Each group of mice ear swelling was measured. Results: 1 analgesic effect of the hot plate test 1.1 SLW 1.43 mg · kg -1 · d -1 pain response latency group was significantly longer than that before treatment, with the model group comparison, the difference was statistically significant (P <0.01); SLW 0.48 mg · kg -1 · d -1 0.16 mg · kg -1 · d -1 extended pain response latency than before treatment group compared with the model group, the difference was not significant sex (P gt; 0.05). Tip SLW 1.43mg · kg -1 · d -1 group has some analgesic effect. 1.2 writhing SLW 1.43 mg · kg -1 · d -1 group of writhing the latency model group, the difference was statistically significant (P <0.01); SLW 1.43 mg · kg -1 · d -1 group and 0.48 mg · kg -1 · d -1 group animals writhing in 20min significantly reduced, compared with the model group, the difference was significant (P <0.01), suggesting that SLW anti writhing role. 2 anti-inflammatory effects test 2.1 acetate abdominal capillary permeability increase experimental model group Evans blue extravasation compared with normal group, the difference was significant (P <0.01). SLW 0.16 mg · kg -1 · d -1 group caused by acetic acid in mouse peritoneal capillary permeability increase has a significant inhibitory effect compared with the model group, the differences There were significant (P <0.05); SLW 1.43 mg · kg -1 · d -1 , 0.48 mg · kg -1 · d -1 group has a significant inhibitory effect on acetate-induced mouse peritoneal capillary permeability increase compared with the model group, the difference was statistically significant (P <0.01). Tip SLW inhibition of acetic acid-induced mouse peritoneal capillary permeability-increasing role. The 0.48 2.2 the ear swelling Law SLW mg · kg -1 · d -1 group can make the mouse ear swelling was significantly lower compared with the model group, the difference was significant (P <0.01). Tip SLW 0.48mg · kg -1 · d -1 group with anti-mouse ear swelling. 2.3 rat paw edema SLW 0.11 mg · kg -1 · d -1 group in the rats after 3,4 h of proinflammatory paw swelling significantly lower compared with the model group, the difference was significant (after inflammation 3h when P <0.01,4 h, P <0.05); SLW 1.02 mg · kg -1 · d -1 < / sup> group in proinflammatory 2,3,4 h after paw swelling can be significantly reduced, compared with the model group, the difference was significant (after inflammation 2,3 h P <0.01,4 h, P < 0.05); SLW 0.34 mg · kg -1 · d -1 group after inflammation 1,2,3,4 h can paw swelling significantly lower compared with the model group, the differences were significant (after inflammation 2,3 h when P for <0.01,1,4 h P <0.05). Prompted SLW 0.34 mg · kg -1 · d -1 group anti-rat paw edema. The 2.4 tampon induced rat granulation tissue formation in experimental SLW 0.34 mg · kg -1 · d -1 0.11 mg · kg -1 · d -1 group can significantly inhibit tampon induced proliferation of granulation tissue, compared with the model group, the difference was statistically significant (P <0.05); SLW 1.02 mg · kg -1 · d -1 group can significantly inhibit the tampon-induced rat granulation tissue, compared with the model group, the difference was significant (P <0.01). Prompted the SLW able to effectively inhibit tampon induced proliferation of granulation tissue. 3 anti-inflammatory mechanism of test 3.1 rat carrageenan inflammation experimental model group of PGE2 in exudate inflammatory exudate PGE2 content in comparison with the normal group, the difference was significant (P <0.01). D -1 -1 · d -1 group and 0.11 mg · kg -1 > group can be significantly inhibited rat carrageenan inflammatory exudate PGE2 levels increased, compared with the model group, the difference was statistically significant (P <0.05); SLW 0.34 mg · kg -1 · d -1 group can be significantly inhibited rat carrageenan inflammatory exudate PGE2 content increased, compared with the model group, the difference was significant (P <0.01). Prompted SLW 0.34 mg · kg -1 · d -1 group is better to reduce the role of PGE2 in the rat carrageenan inflammatory exudate. Experimental model group the 3.2 rat carrageenan inflammatory exudate Hist content inflammation exudate the Hist content with the normal group, the difference was statistically significant (P <0.01). SLW 1.02 mg · kg -1 · d -1 , 0.34 mg · kg -1 · d -1 , 0.11 mg · kg -1 · d -1 group can significantly inhibited rat carrageenan inflammatory increased exudate Hist content, compared with model group , the difference was statistically significant (P <0.05). Prompted SLW 1.02 mg · kg -1 · d -1 , 0.34 mg · kg -1 · d -1 , 0.11 mg · kg -1 · d -1 group is preferred to reduce the content in the role of the rat carrageenin inflammation exudate Hist. 3.3 rat carrageenan inflammatory exudate in 5-HT content of the experimental model group inflammatory exudate 5-HT content compared with normal group, the difference was significant (P <0.01). SLW 1.02 mg · kg -1 · d -1 , 0.34 mg · kg -1 · d -1 groups can be significantly inhibited rat carrageenan inflammatory exudate increased 5-HT content compared with the model group, the difference was significant (P <0.01). Prompted SLW 1.02 mg · kg -1 · d -1 , 0.34 mg · kg -1 · d -1 group, preferably the reduction of the content in the role of 5-HT in the rat carrageenan inflammation exudate. 3.4 adrenalectomized mouse ear swelling SLW 0.48 mg · kg -1 · d -1 group allows the mouse ear swelling was significantly reduced compared with the model group, The difference was statistically significant (P <0.05). The prompted SLW 0.48 mg · kg the the -1 · d -1 group with anti-mouse ear swelling, and its anti-inflammatory effect does not depend on the adrenal exist. Conclusion: SLW has significant analgesic and anti-inflammatory effects, its mechanism may be related to the reducing of PGE2, Hist, 5-HT generate.
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