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Objective: Irritable bowel syndrome (irritable bowel syndrome, IBS) is a result of intestinal stress due to the high sensitivity of the intestinal disease, including abdominal pain, bloating, diarrhea, etiology and pathogenesis has not yet been clarify the functional bowel disorder syndrome. With the accelerating pace of life and society is becoming increasingly fierce competition at home and abroad incidence rising, seriously affecting people's normal work, study and life. The etiology and pathogenesis of IBS has not yet been elucidated. The currently increased visceral sensitivity in IBS is the most important pathophysiological features one basis and symptoms in patients with IBS symptoms diversity. Recent studies suggest that mast cells play a key role in the pathophysiology of intestinal stress disease process, mast cells are activated and release of bioactive media, media release can be further enlarged to a variety of physiological and pathological reactions leading to the occurrence of a variety of symptoms. Therefore, by inhibiting the intestinal mast cell activation, to reduce the release of histamine, the quantity of the medium such as substance P, is the key for treating intestinal stress disease. IBS pathogenesis is complex and involves the spirit, diet, and the individual quality aspects of a single drug clinical role limitations, can not meet the clinical requirements. Traditional medicine also considers that the complex causes of abdominal pain, diarrhea, Governing Law. Huazhuo detoxification soup is a famous traditional Chinese medicine expert Li Dian Gui Professor by years of clinical practice summarize out of by the parties, and by Pulsatilla, Coptis, Scutellaria, Ginseng, Astragalus, Atractylodes, white peony root, dried tangerine peel, windproof, ash bark, flavescens, woody, barley, angelica, licorice, etc., has Huazhuo detoxification, liver and spleen, the effect of the heat dampness, Huazhuo detoxification party over a long period of clinical observation of IBS has a good effect. In order to further investigate the pharmacological treatment of IBS Huazhuo detoxification party and its mechanism, the study on visceral sensitivity of IBS rat model of behavioral and electrophysiological indicators, monitoring of blood biochemistry, trying to explore Huazhuo detoxification party efficacy for IBS and mechanism of action, combined with Huazhuo detoxification party in the practical application of the clinical efficacy studies, and more ways to play a Chinese name Fang, multi-target and overall therapeutic advantage, scientific development and utilization of traditional Chinese medicine name provided the scientific basis, as well as new drugs development and lay a foundation. Methods Experiment: IBS rat model of chronic stimulation of colonic acetate production visceral hypersensitivity. The modeling is divided into the juvenile rat colonic acetate chronic stimulation group (NA group), adult rat colonic the chronic irritation acetate group (AA group) and blank control group (NS). Modeling groups IBS rat model for assessment of visceral sensitivity (abdominal electrical activity and abdominal retraction reflex), to verify whether the model successfully. Experiment II: To investigate the effect and mechanism of Huazhuo detoxification party visceral hypersensitivity in a rat model of IBS. After the modeling, the experimental animals were divided into control group (A), model control group (B), Shute group (C group), Huazhuo detoxification the party low dose group (D group), Huazhuo detoxification The party high dose group (E). Observe the rats in each group under the influence of different factors intestinal distension abdomen raised and back arched capacity threshold and rat intestinal capacity expansion during abdominal wall contractions, detection of 5 - hydroxytryptamine (5-HT), P substance (SP), calcitonin gene-related peptide (CGRP) content. The results of the experiment: in the first six weeks of the modeling, NS group of eight rats cause abdominal elevation and back arched expansion capacity threshold were (0.70 ± 0.10) ml and (0.94 ± 0.16) ml AA rats two threshold (0.76 ± 0.06) ml and (1.02 ± 0.88) ml, no significant difference between sex (P gt; 0.05). And the the NA rats abdomen elevation and back arch threshold were (0.43 ± 0.06) ml and (0.61 ± 0.08) ml, a significant difference compared with the first two groups (P = lt; 0.01); at 0.5 ml capacity, NS group and AA rats abdominal wall contraction is not obvious, and the the NA rats abdominal wall contraction number up to (6.63 ± 1.06) / 5min, the difference was statistically significant (P lt; 0.01). In 0.8ml expansion of capacity, the the NA rats abdominal wall contractile activity than those in the other two groups is more significant (P lt; 0.05). Rat abdominal wall contraction in the the 1.2ml expansion capacity NA rats compared with NS group was significantly increased (P lt; 0.05), no significant differences with the AA group sex (P gt; 0.05). Experiment II: In behavioral tests, medication, B group compared with group A, causing the rat abdomen lift and back arched expansion capacity threshold is significantly lower, a significant difference (P lt; 0.01, P lt; 0.05) compared to groups C, D, E and A group, there was no significant difference (P gt; 0.05); compared to the D, E and C groups, there were no significant differences (P gt; 0.05); abdominal electrical activity recorded The results showed that after treatment, B group compared with the group A, 1.0ml, 1.5ml expansion of capacity, the number of rat abdominal wall muscle contraction significantly increased, and the difference was significant (P lt; 0.01), while the C, D, E group compared with group A, respectively, without significant differences (P gt; 0.05), D, E and group C group, there was no significant difference (P gt; 0.05); compared with the model group, the treatment group mouse behavioral and electrophysiological parameters were significantly improved; each treatment group rat model of 5-HT, SP content decreased of CGRP content increased, and there is a dose-effect relationship. Conclusion 1. Using acetate can cause chronic stimulation of colon stable intestinal the high sensitivity IBS rat model; 2. Huazhuo detoxification can decrease the IBS model rat serum 5-HT, plasma SP content, increased CGRP levels, large doses Huazhuo detoxification party more effective than Shute. 3. Huazhuo detoxification mechanism of action of the party may be lower model serum levels of 5-HT, plasma SP content weakened the excitability of dorsal horn neurons, visceral pain threshold to eliminate intestinal allergy, so as to achieve therapeutic purposes. 4 the. Huazhuo detoxification side lies not only has Huazhuo detoxification, liver and spleen, the effect of the heat dampness, can regulate immune function, improve microcirculation, scavenging oxygen free radicals, inhibition of the inflammatory response, analgesic role . Seen Huazhuo detoxification side treatment of IBS is multi-faceted, multi-link, the combined result.
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