Dissertation > Excellent graduate degree dissertation topics show

Serum homocysteine ​​, endothelial microparticles and the pathogenesis of preeclampsia

Author: HuangHuaPing
Tutor: ZhangHong
School: Suzhou University
Course: Obstetrics and Gynaecology
Keywords: homocysteine endothelial microparticles Preeclampsia endothelial injury liquid chromatography-tandem mass spectrum flow cytometric analysis
CLC: R714.245
Type: Master's thesis
Year: 2011
Downloads: 28
Quote: 0
Read: Download Dissertation

Abstract


Objective:To investigate the serum homocysteine (Hey), endothelial microparticles (EMPs) in preeclampsia, compare the variation prepartum and postpartum.Methods:27 women of preeclampsia (severe preeclampsia 17 cases, mild preeclampsia 10 cases) and 19 normal term pregnant women were collected. Their peripheral blood was respectively collected within 24h after their admission and the third day after Cesarean section. The serum Hey was detected by liquid chromatographic-tandem mass spectrometry (MS) detection (LC-MS/MS). And the levels of corresponding serum EMPs were detected by flow cytometric analysis. The relevance of concentrations of Hey and EMPs with mean arterial pressure (MAP) and urine protein were analysed.Results:1. The serum Hey in the preeclampsia group was significantly higher than that of the normal term pregnant group (10.97±3.32 umol/L.vs.7.28±2.17 umol/L, P<0.01), The serum Hey of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (12.10±2.91 umol/L.vs.8.82±3.08 umol/L, P<0.01), The serum Hey of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). The postpartum serum Hey of the preeclampsia group was significantly higher than that of the normal term pregnant group (10.17±2.40 umol/L.vs. 7.68±2.08 umol/L, P<0.01). The postpartum serum Hey of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (10.90±2.47 umolL.vs. 8.79±1.58 umol/L, P<0.05). The postpartum serum Hcy of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). In every group, the prepartum serum Hcy had no significant difference compared with the postpartum serum level (P>0.05). 2. The serum EMPs of the preeclampsia group was significantly higher than that of the normal term pregnant group (2863.5±1543.1/ml.vs.1703.0±592.2, P<0.01). The serum EMPs of the severe preeclampsia group was significantly higher than that of the mild preeclampsia group (3467.5±1440.1/ml.vs.1836.7±1153.8/ml, P<0.01). The serum EMPs of the mild preeclampsia group had no significant difference compared with that of normal term pregnant group (P>0.05). The postpartum serum EMPs had no significant difference in every group. The prepartum serum EMPs of was significantly higher than the postpartum serum level in the preeclampsia group and the severe preeclampsia group (2863.5±1543.1/ml.vs.1262.0±685.8/ml, P<0.01; 3467.5±1440.1/ml.vs.1421.8±760.2/ml, P<0.01). The prepartum serum EMPs had no significant difference compared with the postpartum serum level in the mild preeclampsia group and normal term pregnant group (P>0.05).3. There was significantly positive correlation and linear regression between serum Hcy and the MAP as well as 24h urine protein of preeclampsia group(R=0.53, P<0.01; R=0.68, P<0.01). similarly, there was significantly positive correlation and linear regression between the level of EMPs in serum and the MAP as well as 24h urine protein of preeclampsia group(R=0.33, P<0.05; R=0.67, P<0.01).4. There was significantly positive correlation and linear regression between the level of Hcy and the EMPs in serum(R=0.59, P<0.01).Conclusion:1. The serum Hcy and EMPs in preeclampsia group was significantly higher than that of the normal term pregnant group. The serum Hcy and EMPs in the severe preeclampsia group was significantly higher than that of the mild preeclampsia group. They had positive correlation and linear regression with the MAP and 24h urine protein. From all of this, it suggests that Hcy and EMPs may be play important role in the pathogenesis of preeclampsia.2. In each group, the postpartum serum EMPs was significantly descended compared with that before termination in the preeclampsia group. It suggested termination of pregnancy can modify endothelial function, and it provided direct evidences to cordinated the theory of endothelial injury in the pathogenesis of preeclampsia.

Related Dissertations

  1. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  2. C-reactive protein,homocysteine ??levels in type 2 diabetic peripheral neuropathy of TCM,R259
  3. Common TCM syndrome and serum homocysteine ??and diastolic function relationships of the newly diagnosed patients with type 2 diabetes,R259
  4. The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction,R542.22
  5. α-Lipoid Acid Prevents Endothelial Dysfunction in HUVEC-12 Via Up-regulation of Lysyl Oxidase,R543.5
  6. The Research of Nf-kB、TNF-a and PLGF in the Pathogenesis and Severity of Preeclampsia and Theirs Relevance,R714.244
  7. The Influence of Common Complications with Pregnancy to the Fetal Blood Flow Velocity Waveforms and the Value,R714.25
  8. The Clinical Study on Relationship between Plasma Homocysteine Levels and Recurrent Cerebral Infarction,R743.3
  9. Changes of the Metalloprotease That Cleaves Von Willebrand Factor in Women During Normal Pregnancy and Other Diseases Like Gestational Hypertension,R714.25
  10. The Discuss of Tei Index and Ejection Fraction of Fetal Cardiac Function in Preeclampsia,R714.25
  11. Changes of Circulating Endothelial Microparticles in Patients with Kawasaki Disease,R725.4
  12. Relationship between Endothelial Cell Derived Microparticles and aGVHD in Allogeneic Hematopoietic Stem Cell Transplantation,R457
  13. The Correlative Analysis between Auto-antibodies Against AT1-Receptor、Autoantibodies Aganinst Aplhal-adrenergic Recetpor and Endothlial Micropartles in Patients with Gradc Three Hypertension,R544.1
  14. The Study on the Relationship between Folic Acid, Homocysteine and Kazak’s Esophageal Cancer,R735.1
  15. The Effect of Hemodialysis and Hemodiafiltration on Endothelial Function in Maintenance Hemodialysis Patients,R692.5
  16. The Association of MTHFR C677T and MS A2756G Polymorphisms and Hcy Level with Male Infertility,R698.2
  17. The Correlative Study of Plasma Hcy, D-D and CRP in Patients with AECOPD,R563.9
  18. The Relationship between the Level of Homocysteine and Important Target Organs Damage in Essential Hypertension.,R544.1
  19. Urotensin Ⅱ Receptor Mediates the Expression of Interleukin-6 in Rat Adventitial Fibroblasts Stimulated by Homocysteine,R543.5
  20. Experimental Study on Effects of Allicin on Proliferative Rabbits’ Vascular Smooth Muscle Cells Induced by Homocysteine,R285.5
  21. Study of the Correlation Between Plasma Hcy, Hs-CRP, IL -6 and Vascular Cognitive Impairment No Dementia after Cerebral Infarction,R749.1

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy ) > Gestosis > Eclampsia
© 2012 www.DissertationTopic.Net  Mobile