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Lentinan (Lentinan, LNT) has a variety of functions, anti-tumor, anti-clotting and immunomodulatory activity is an excellent natural source of edible and medicinal active ingredient, has broad application prospects. Lentinan for the study, the effects of the mushrooms submerged fermentation lentinan process conditions to study the the lentinan phosphorylation and lentinan and Zn 2 chelating optimal process and product physical and chemical properties analysis. The main results are as follows: 1, to determine the process conditions in submerged culture mushrooms producing extracellular polysaccharide. Mushroom culture after 8 days, the extracellular polysaccharides reached the maximum 0.476g/100mL; the mushrooms were further cultured to 10 days, the biomass reaches a maximum value 1.233g/100mL. Further experiments investigated the influence of the medium mushrooms submerged fermentation results showed that the yield of glucose, peptone and NaCl three factors on extracellular polysaccharides have a significant effect, the optimum carbon source is glucose medium, the optimum nitrogen source for peptone. When glucose, peptone, NaCl, respectively, for the 25 g / L, 16g / L, 0.8 g / L, the extracellular polysaccharide content 0.8460g/100mL. 2 determined by orthogonal experiments the lentinan phosphorylated. Select mass fraction of 8% sodium tripolyphosphate (STPP) and the partial sodium (STMP) mixture as phosphorylating agents, control the temperature 80 ℃, pH8.0 conditions reaction for 5 hours. The test results show that phosphorylation lentinan phosphate derivative graft amount of 7.771%, the viscosity measurement show that lentinan after phosphorylation, almost no change in viscosity. 3, of lentinan and Zn 2 chelating optimum reaction conditions were as follows: mass fraction of 25% the ZnSO of 4 · 7H 2 O temperature 90 ° C, pH 7.0, time 4h. In the process conditions, lentinan Zn 2 was increased by 9.7363 percent. 4, the product of deep fermentation lentinan modified analysis and structural characterization of physical and chemical properties. The results show that the extracellular polysaccharide, phosphorylation of lentinan and the Zn 2 chelating lentinan are soluble in water, containing a reducing sugar, monosaccharide or sugar, no starch material. Extracellular polysaccharides, polysaccharides and uronic acid contents were 86.25-88.40% and 4.298-4.952%. Lentinan by phosphorylation and Zn the 2 chelation, polysaccharides and uronic acid content varied slightly, lentinan structural modification, its structure is almost not been destroyed. 5, antioxidant activity experiments showed that lentinan has antioxidant activity, and phosphorylation of and Zn of 2 chelating lentinan compared with the antioxidant activity of unmodified. Anti-complement experiments show that phosphorylation of lentinan anti-complement activity than unmodified lentinan.
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