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Objective: To measure levels of serum resistin in patients with nonalcoholic fatty liver disease (NAFLD). To investigate the relationship between serum resistin and insulin resistance, and the role of serum resistin in the pathogenesis and progression of NAFLD.Methods: 53 patients (including 30 males and 23 females) with NAFLD and the 28 control subjects (including 16males and 12females) were enrolled in this study. The height, weight, waist circumstance, hip girth and blood pressure of all subjects were obtained by standard methords. To all the subjects, the parameters including fasting blood glucose(FBG), triglyceride(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-c), low-density lipoprotein cholesterol (LDL-c), alanine aminotransferase(ALT), aspartate aminotransferase(AST)and gamma glutamyl transferase(GGT)were measured. Serum resistin were measured by electrochemiluminescence immunoassay (ECLIA) and fasting serum insulin (FINS) were measured by enzyme-linked immunosorbent assay (ELISA). Body mass indexes (BMI), waist hip ratio (WHR), body fat percentage (BF%) were calculated with formula. Homeostasis model assessment was applied to assess the status of insulin resistance index (HOMA-IR). The relationships between serum resistin and various parameters in patients with NAFLD were observed and analyzed by statistical methods.Results: 1. There were no significant differences in the number of cases, average age, compositions of sex and BF% between the NAFLD group and the control group (P > 0.05). In the NAFLD group, systolic blood pressure (SBP), diastolic blood pressure (DBP) and WHR were higher than those of control group (P = 0.001). The waist circumference, hip girth and BMI in NAFLD group were significantly higher than those of control group, and there was significant difference between two groups (P < 0.0005).2. There were no significant differences on the level of TC, HDL-c, LDL-c between the NAFLD group and the control group (P > 0.05). In the NAFLD group, FBG, TG, AST were higher than those of control group (P = 0.001, P = 0.004, P = 0.001). The level of serum resistin, FINS, HOMA-IR, ALT and GGT in NAFLD group were significantly higher than those of control group, and there was significant difference between two groups (P < 0.0005).3. There was no significant correlation between serum resistin and age, BMI, WHR, BF%, FBG, TG, TC, HDL-C, LDL-C, AST in NAFLD group (P > 0.05). The levels of serum resistin in patients with NAFLD were positively correlated with GGT, ALT, FINS, HOMA-IR (r = 0.354, P = 0.009; r = 0.391, P = 0.004; r = 0.875, P < 0.0005; r = 0.881, P < 0.0005 respectively).4. After multiple stepwise regression analysis, the results showed that HOMA-IR was the most important factor for affecting the levels of serum resistin.5. The levels of serum resistin in male and female patients with NAFLD were higher than those of control group (P < 0.05). But no statistical difference was found between males and females in two groups (P > 0.05).Conclusions: 1. The levels of serum resistin in patients with NAFLD were significantly higher than those of controls.2. Serum resistin in patients with NAFLD was closely correlated with insulin resistance, and it may participate in the pathogenetic progresss of NAFLD.3. Serum resistin in patients with NAFLD might be associated with hepatic insulin resistance, and it has no correlations with obesity-related insulin resistance.4. Serum resistin in patients with NAFLD may participate in the inflammation of fatty liver disease as inflammatory cytokines.
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