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Study on the Hydrolysis of Soy Protein Isolate in an Enzymatic Membrane Reactor

Author: WangWenJie
Tutor: WangZuo
School: Jiangnan University
Course: Of Food Science
Keywords: Enzyme membrane reactor Protease N Soy protein isolate Response surface analysis Degree of hydrolysis Antioxidant activity ACE inhibitory activity
CLC: TS201.2
Type: Master's thesis
Year: 2008
Downloads: 239
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Abstract


The Enzymatic Preparation has the biological activity of the protein hydrolyzate and its application in the food industry is being more and more attention. Hydrolysis of the traditional way is to use a batch-type, in this manner, however, the existence of a number of disadvantages. Combined ultrafiltration / nanofiltration membrane technology and hydrolysis reactor can achieve continuous separation of small molecular weight hydrolyzate hydrolysates, this method more and more attention. This article will cover isolated soy protein hydrolysis enzyme membrane reactor. First, Alcalase 2.4L (EC 3.4.21.62), alkaline protease and neutral protease, Protease N (IUB 3.4.24.28) hydrolysis of the soy protein isolate (SPI) and hydrolysis characteristics were studied in a batch reactor. The results show that: Protease N has high proteolytic activity, optimum temperature and good stability, the hydrolysis process susceptible to product inhibition; relative molecular mass of the hydrolysis products of the two enzymes were less than 10,000, the low levels of free amino acids ; Protease N is more suitable for the hydrolysis of the SPI enzyme membrane reactor Protease N as the latter enzyme membrane reactor with enzyme. To obtain the optimum conditions of the hydrolysis of SPI enzyme membrane reactor, the average moisture flux Javerage, the protein recovery Sapparent well as an index via the liquid hydrolysis degree DHpermeate, response surface analysis methods on the initial moisture flux Ji OF early starting protein concentration [S] and the initial concentration of the protease [E] / [S] is optimized. The results indicate that the the three factors Javerage, Sapparent and DHpermeate significant, and there are significant differences. The optimum conditions optimized to: Ji 10mL/min, [S] at 4% (w / v), [E] / [S] 1.5% (w / w), under this condition Javerage, Sapparent, and DHpermeate respectively 71.20%, 42.55% and 32.35%. In order to confirm the effect of the enzyme membrane reactor, on the physical and chemical properties and functional properties of hydrolyzate and macroporous resin static desalting elution and ethanol fractionation. The results show that: the hydrolysis product having a good solubility and a low viscosity, but also has good antioxidant activity. The macroporous resin hydrolyzate salt can be completely removed, use 25%, 50%, 95% ethanol solution static desorption the desalination rate were 90.72%, 92.89% and 93.29%, protein recoveries were 42.37 %, 28.16% and 15.98%, the antioxidant activity of the desalted hydrolyzate and ACE inhibitory activity have been enhanced and increases with the increase of ethanol concentration was eluted. Wherein, ACE inhibitory activity IC50 value is reduced to the 95% ethanol elution by desalting before 0.418mg/mL 0.104mg/mL.

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