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After the wort flat natural selection , screening purified trispora (Blakeslea trispora) strains of the genetic characteristics of stable high yield of lycopene . Study trispora shake flask batch fermentation conditions . In the fermentation medium single factor affecting the study on the basis of the test , the first use of the Plackett-Burma designed to filter out the most important of several factors ; then apply the steepest ascent method approximation lycopene production areas , and finally the use of response surface analysis of the test method determine the optimal level of three important factors , and fermentation conditions . Conditions of this optimization , the final shake flask fermentation yield of lycopene to 1.24 g / L , and the fermentation yield 0.8g / L, a 55% increase over the initial conditions . Meanwhile , from the perspective of comprehensive utilization of amino acids of the body of the three spore Brad mold quantitative analysis , development, nutrition basis for the development of high-quality feed . In the Blakeslea trispora the biosynthesis of lycopene metabolic pathways in research to explore the effect of various metabolic pathways , a key enzyme in the biosynthesis of lycopene . Accelerator , in order to increase the ability of cell growth and production of color by adding a key enzyme activity . The study showed that: Reagent A, penicillin, surface active agent (span-20 , etc.) , an oxygen carrier ( n - hexane , H2O2) and three spore acid structure analogue (β- ionone ) and beyond because of trispora fermentative production of lycopene has a very large role in promoting . Wherein reagents A, penicillin , beta - violet Luo ketone, etc. The promoting effect of respectively 31% , 32% and 35% ; for the oxygen carrier and a surfactant , n-hexane and effect is not obvious , while the optimum addition of H2O2 and Span20 the time and concentration were : 48h , 0.05 % 0h , 0.1 % , relative to the blank were increased by 16.7% and 22.7% . The present study the Blakeslea trispora wet cells of various pretreatment methods on the extraction of purified lycopene . Experimental studies have shown that : the enzyme by saponification plus ethanol pretreatment , extraction and purification of lycopene effect is better , at the same time can reduce the extraction and purification of the drying step , provide the economic base for the industrialization and mass production . As for the the lycopene extraction process , the results showed that: acetone as a solvent to extract the best , the best conditions : material ratio ( mycelium and acetone , mass volume ratio , g / mL) 1:200 , temperature 40 ℃ time of 2h.
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