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Studies on the Isolation, Purification and Structure of the Pigment in Greening Garlic

Author: ChenCong
Tutor: QiaoXuGuang
School: Shandong Agricultural University
Course: Of Food Science
Keywords: Garlic Green pigment Separation and purification Structure Nature
CLC: TQ914
Type: Master's thesis
Year: 2008
Downloads: 260
Quote: 6
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Abstract


Garlic (Allium sativum L.), the Liliaceae onion genus. Garlic bulbs with a high nutritional value, containing protein, carbohydrates, fat, vitamins and calcium, phosphorus, iron, selenium, germanium and other mineral elements. China is a major producer of garlic, with its output accounting for three-quarters of the world's total output. In addition to fresh, garlic is often processed into garlic, garlic, garlic flakes, garlic powder and other products, and often green phenomenon but in the process, seriously affected the quality of the product's appearance, become impede further processing of garlic major problems. Therefore, to research the garlic products greening the mechanism, effectively control the a greenish phenomenon, to achieve large-scale production has important significance. In this experiment, the garlic bulb as raw material, crude extracts prepared by extraction garlic green pigment, solid phase extraction, adsorption resin and gel chromatography Isolation and Purification of the garlic green pigment; using LC-MS Determination of the molecular weight of the garlic green pigment technology, and nuclear magnetic resonance, infrared spectroscopy for structural analysis of pigment samples; garlic green pigment light stability, the mechanism of garlic greening provides a theoretical basis. 1 garlic greening pigment separation and purification of the test is to establish a high-performance liquid chromatography (HPLC) separation of detection garlic greening pigment to determine the detection garlic greening pigment chromatographic conditions are as follows: Alltech Alltima C18 (250mm × 4.6 mm) column, mobile phase of acetonitrile: water = 2:8 (containing 0.1% formic acid), the column temperature was 30 ° C, detection wavelength of 440 nm, flow rate 1.0mL/min. In the liquid chromatography under the conditions of the two substances in garlic green pigment retention time of 2.89min and 3.95min. On this basis, using a variety of column chromatography to garlic greening pigment study of separation and purification, and high-performance liquid chromatography to test their effectiveness. The first step purification by solid phase extraction and adsorption resin compared, then the best column material from the the four SPE cartridges and two macroporous resin XAD-16 macroporous adsorption resin. The experiments showed that the The garlic green pigment crude extracts by XAD-16 macroporous resin, with methanol containing 0.2% HCl elution flow rate 1BV / h, the green pigment collected effluent purification better. Second purification using a Sephadex LH-20 gel resin was separated and purified using pure methanol as the eluent, flow rate 1.2BV / h, get a sample of higher purity. (2) the determination of molecular weight and structure of the qualification test of the green pigment substances by LC-MS technique Determination of the molecular weight of the substance of the green pigment, the results showed that the green pigment molecular weight of 527. The green pigment solid samples prepared at the same time, their structures were identified by nuclear magnetic resonance and infrared spectroscopy. NMR initially identified the green pigment material: (1) pyrrole ring, and on the H-containing substituents ② unsaturated bond, C = C ③ methyl. Infrared spectroscopy initially identified the green pigment substances: (1) belongs to the aromatic heterocyclic compounds, suggesting that N-containing pyrrole derivatives. ②-CH3,-OH, C = C and C = S. 3 the garlic green pigment light stability study using the measured suction-light value and the UV-visible scan method to study the influence of of various nature light on the stability of the pigment, the test results show that the poor tolerance of the green pigment of the light, outdoor natural light and UV pigment, UV its most significant impact. Light green pigment blue pigment component completely disappeared, the yellow pigment component decreased.

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