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The Expression and Significance of BMP2mRNA in Regeneration of Rat Liver Tissue

Author: JiWenMin
Tutor: XuCuiPing
School: Shanxi Medical
Course: Digestion within the science
Keywords: Liver regeneration BMP2mRNA NF-κB In situ hybridization
CLC: R575
Type: Master's thesis
Year: 2008
Downloads: 38
Quote: 0
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Abstract


Objectives:1.at the level of genes, the expression of BMP2 in liver tissues and the characteristics of dynamic changes in the process of liver regeneration will be observed. 2. Combined with the characteristics of expression changes of NF-kB in the process of liver regeneration, the impact of BMP2 on the process will be explored.Methods: Healthy and male Wister rats were randomly divided into 3 groups—normal control group (NC) i.e. 0h group, sham-operated group (SO) and partial hepatectomy (PH). The expressions of BMP2mRNA in liver tissues of NC, SO and PH after 6h, 12h, 24h, 48h were observed using situ hybridization method; Immunohistoche-mistry method was adopted to determine the expressions of NF-kB in liver tissues of several groups said earlier.Results: Through the Image Analysis System and the Statistical Analysis, the following results can be shown: 1. the expression of BMP2mRNA exists in rat liver tissues of NC; 2. there is no significant difference in the expression levels of BMP2mRNA in rat liver tissues between SO group and NC group; 3. there exist statistical significance(p<0.05)comparing the expression of BMP2mRNA in rat liver tissues of PH after 6h, 12h, 24h, 48h with the BMP2mRNA expression of NC group and SO group in the corresponding moments; 4. there exists statistical significance (p<0.05) among the expression rats of PH groups after 6h, 12h, 24h, 48h; 5. there is no significant difference expression of BMP2mRNA of between PH group after 48h and NC group; 6. there is no NF-kB expression in liver tissues of NC group and SO group; 7. there exist statistical significance(p<0.05)comparing the expression of NF-kB in rat liver tissues of PH group and the NC group and SO group at different time points; 8. among the NF-kB expression rates of PH groups after 6h, 12h, 24h, 48h exists statistical significance (p<0.05); 9. through correlation analysis about expression rates of BMP2mRNA and NF-kB, such conclusion can be drawn that BMP2 and NF-kB are negatively correlated(r =-0.860, p<0.05)in the early period of liver regeneration and are positively correlated(r =0.821, p<0.05)in the latter period of liver regeneration.Conclusion: 1. there exists the expression of BMP2 in normal rat liver tissues and the change in the process of liver regeneration; 2. there is no NF-kB expression in normal rat liver tissues,NF-kB has the expression in the process of liver regeneration and changes in strength and position of its expression take place with the progress of liver regeneration’s time; 3. BMP2 may inhibits liver regeneration.

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Liver and gall bladder disease
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