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Effects of Cyclosporin A on Expression of Anti-M2-muscarinic Acetylcholine Receptor Autoantibody in Asthmatic Rats

Author: WangBaoJun
Tutor: LiuXueJun
School: Shanxi Medical
Course: Geriatrics
Keywords: Bronchial asthma M2 receptor autoantibodies Airway hyperresponsiveness Airway inflammation Cyclosporin A
CLC: R562.25
Type: Master's thesis
Year: 2008
Downloads: 18
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Abstract


Objective: To detect asthma rat serum M 2 the receptor concentration autoantibodies to explore M 2 receptor autoantibodies with asthma relationship. Changes in rat airway inflammation and airway hyperresponsiveness observed by intraperitoneal injection of cyclosporine A (CsA), asthma, and on M 2 receptor autoantibody concentration of cyclosporine A role in the treatment of asthma, and to further explore the pathogenesis of asthma airway hyperresponsiveness. Methods: 39 healthy male Wistar rats were randomly divided into a control group (A), asthma model group (B), the CsA intervention group (C group), each group of 13. Applications chicken ovalbumin sensitization and aerosol inhalation asthma rat model using the ELISA method to detect the rat serum M 2 receptor autoantibodies content; bronchoalveolar lavage fluid (BALF ) in the total number of inflammatory cells, the absolute number of eosinophils (EOS), EOS percentage; applications airway pressure time index (APTI) said that the airway responsiveness by measuring the APTI values ??reflect changes in rat airway reactivity; and detect intraperitoneal injection of cyclosporine A in asthmatic rats each index to determine its effect on asthma treatment. Results: Asthma serum M 2 receptor autoantibody titers (1:120) and antibody-positive rate (83.33%) and the control group (1:20; 8.33%), and CsA group (1:35; 25.00%) compared to the difference was significant (P <0.01); CsA group compared with the control group, the difference was not statistically significant (P> 0.05). WBC (35.34 ± 0.77 × 10 4 / ml) in the BALF of asthmatic rats and EOS percentage (23.45% ± 0.77) was significantly higher than that of the control group (8.44 ± 0.39 × 10 4 / ml; 0.70% ± 0.06), (P <0.01), and the difference was statistically significant compared with the CsA group (24.43 ± 0.67 × 10 4 / ml; 11.44% ± 0.31) (P <0.01); CsA group and the control group (P <0.05), there is still a difference. The asthma group APTI value compare with the control group, CsA group, mACh concentration of 0.5 mg / kg, three mean were 0.504 ± 0.143,0.277 ± 0.161,0.416 ± 0.150 mmHg / s; mACh concentration of 1.0 mg / kg, mean, respectively, for the three 0.617 ± 0.10 8 , 0.434 ± 0.128,0.541 ± 0.117 mmHg / s; the mACh concentration of 1.5 mg / kg, three mean were 0.794 ± 0.073,0.463 ± 0.120, 0.676 ± 0.092 mmHg / s; difference was statistically significant (P <0.01); CsA group and the control group (P <0.05), there is still a difference. Conclusion: Asthma serum M 2 receptor autoantibodies were highly expressed, and M 2 receptor autoantibodies may be involved in the pathogenesis of asthma and asthma gas a factor of airway inflammation and airway hyperresponsiveness. Cyclosporine A can significantly reduce of asthmatic rats M 2 receptor autoantibody expression significantly reduced airway inflammation and airway hyperresponsiveness, so as to achieve the purpose of the treatment of asthma.

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CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Trachea and bronchial disease > Bronchial disease > Bronchial asthma
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