Dissertation > Excellent graduate degree dissertation topics show

Effect of NK-1 Receptor on p38MAPK Activasion in Spinal Cord of the Formalin Test Rats

Author: JinXing
Tutor: ChengQiao；ZhangCe
School: Shanxi Medical
Course: Anesthesiology
Keywords: NK-1 receptor p38MAPK RP67580 formalin
CLC: R402
Type: Master's thesis
Year: 2008
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


Objective:To assess the effect of NK-1 receptor on rat’s behavior response and p38MAPK activasion in spinal cord.Methods:The experiment is divided into two parts.The first part is the peripheral test.Investigation of rat’s behavior:24 male Wister rats were randomly divided into 4 groups(n=6 each).Including control group(DMSO) and three different dose of RP67580 groups:5nmol;20nmol;100nmol.All groups were injected medicine(or dissolvent) into the dorsal surface of the left hind paw,after 15 min,injected 5%formalin 50ul into the same site,then observed the behavior and calculated the time of licking or biting injected paw every 3 min for 1 hour.Western blot test:The groups were as the same as the test before,the spinal p38 MAPK identified by the Western Blot technique after the injection of formalin 20 min.The second part is the center test:Investigation of rat’s behavior:24 male Wister rats which operated successfully were randomly divided into 4 groups(n=6 each).Including control group(DMSO) and three different dose of RP67580 groups:5nmol;20nmol;100nmol.All groups were injected medicine(or dissolvent) intrathecally,after 15 min,injected 5%formalin 50ul into the same site,then observed the behavior and calculated the time of licking or biting injected paw every 3 min for 1 hour.Western blot test:The groups were as the same as the test before,the spinal p38 MAPK identified by the Western Blot technique after the injection of formalin 20 min.Result1.Effect of peripheral NK-1 receptor on p38MAPK activation in spinal cord of the formalin test ratsInvestigation of rat’s behavior:The group of 5nmol and 20nmol had no effect on the pain reaction in phaseⅠbut had effect in phaseⅡ,the time of licking or biting was shorter than the control group;The group of 100nmol had effect on it in phaseⅠandⅡ,the time of licking or biting was shorter than the control group(p＜0.05).Western blot test:Compared with the control group,the expression of phosphorylated p38MAPK(P-p38) in spinal cord in all experiment groups were decreased(P＜0.01). 2.Effect of spinal NK-1 receptor on p38MAPK activation in spinal cord of the formalin test ratsInvestigation of rat’s behavior:The group of 5nmol had no effect on the pain reaction in phaseⅠbut had effect in phaseⅡ,the time of licking or biting was shorter than the control group;The group of 20nmol and 100nmol had effect on it in phaseⅠandⅡ,the time of licking or biting was shorter than the control group(p＜0.05).Western blot test:Compared with the control group,the expression of phosphorylated p38MAPK(P-p38) in spinal cord in all experiment groups were decreased(P＜0.01),and the difference between experiment groups was significant(P＜0.05).The expression of P-p38 was decreased significantly with the increase of medicine dosage.Conclusions:1.Peripheral or intrathecal administration of NK-1 receptor antagonist RP67580 could inhibit the rat’s pain response induced by injection of formalin.2.Peripheral or intrathecal administration of NK-1 receptor antagonist RP67580 could inhibit the activation of p38MAPK in spinal cord induced by injection of formalin.3.The effect of NK-1 receptor on the rat’s pain response at least partly mediated by the p38MAPK pathway.

Related Dissertations

  1. The Effects of Atorvastatin on Expression of Osteopontin in Kidney of the Diabetic Rats Its Molecular Mechanism,R587.2
  2. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  3. Expression and Correlation of Urokinase-type Plasminogen Activator (uPA) and Its Receptor (uPAR) and Mitogen Activated Protein Kinase (MAPK) p38 in Endometrial Carcinomas,R737.33
  4. Down-Regulation of Spinal D-Amino Acid Oxidase Expression Blocks Chronic Pain,R96
  5. P38MAPK Signal Pathway Modulated Proliferation, Apoptosis and Cell Cycle of Rat Hepatic Stellate Cells Stimulated by Acetaldehyde,R575.2
  6. The Effects of Chloride Channels Blockers on the GABA-Activated Current in Acutely Dissociated Rat DRG Neurons,R96
  7. Intrathecal tramadol on inflammatory induced pain behavior and spinal c-Fos protein in,R96
  8. The Effects and Mechanisms of Simvastatin on High Glucose-induced Injury in the Neonatal Rat Cardiomyocytes,R965
  9. The Study on the Role of p38MAPK in TRAIL-induced Apoptosis of Multiple Myeloma and Its Relationship with CXCR4,R733.3
  10. The Effects of Caspase-3 Inhibitor on the Neuronal Apoptosis of Rats Spinal Cord and Hyperalgesia Induced by Formalin Inflammatory Pain,R114
  11. Study on Preemptive Analgesia Effecs of Parecoxib Sodium of Injection of IL-6 and COX-2 in the Formalin Test Rats,R965
  12. Antinociception of Intrathecal Dexmedetomidine in the Mouse Formalin Test,R614
  13. Experimental Study on the Activation of P38 Mitogen Activated Protein Kinase When Low Intensity Pulsed Ultrasound Promoted Human Periodontal Ligament Cells Osteogenic Differentiation,R781.42
  14. Research on Expression of NK Cell Receptors/Ligands in Acute Leukemia Patients and Its Relationship with GVHD and Relapse after Haploidentical Hematopoietic Stem Cell Transplantation,R733.71
  15. Effect of SB203580 on Apoptosis and Expression of BCL-2 Protein in Rat Hepatic Stellate Cells,R575.2
  16. Eeffcts of Preemptive Intrathecal Clonidine on Behavior and Expression of Pcreb Protein in the Spinal Cord of the Formalin Test Rats,R96
  17. Effects of Losartan on Renal p38 MAPK Signal Transduction Pathway in IgA Nephropathy Rats,R692.3
  18. Effect of Intrathecal Clonidine on C-fos Expression in Dorsal Horn of Spinal Cord in Formalin Inducing Rats Pain,R614
  19. Increased Expression of HO-1 on Spinal Cord during Formalin Inflammatory Pain and the Role in Neuronal Apoptosis,R402
  20. Effects of Intrathecal Esmolol on Nociception and Spinal c-Fos, COX-2 mRNA Expression in the Mouse Formalin Test,R96
  21. A Study of Analgesic Effect and Mechanism of Crystal-8 from Zanthoxylum Nitidum(Roxb.)Dc.,R285.5

CLC: > Medicine, health > Clinical
© 2012 www.DissertationTopic.Net  Mobile