Dissertation > Excellent graduate degree dissertation topics show

Separation and Analysis of Polysaccharide from Prunella Vulgaris Linn

Author: HaoGuiTang
Tutor: CaoYuHua；DaiJun
School: Jiangnan University
Course: Analytical Chemistry
Keywords: Prunella vulgaris Linn Polysaccharides Extraction Purification Monosaccharide composition Pre-column Derivatization P-Aminobenzoic acid High Performance Liquid Chromatography
CLC: R284
Type: Master's thesis
Year: 2007
Downloads: 417
Quote: 2
Read: Download Dissertation

Abstract


Prunella vulgaris Linn is the dry spike of Prunella vulgaris Linn which belongs to self-heal category, labiate .Its name backs from the saying“the grass will die away after the Summer Solstice”.Now, research show the Prunella vulgaris Linn polysaccharides has many functions such as antivirus ,antibacterial,lower down blood pressure,antiinflammation, reducing blood fat, anticancer, restrating immunity and so on. The Prunella vulgaris Linn polysaccharides has gotten more and more attention on clinic value and exploitation value, just for it is a kind of potential resource.In this paper, we used Prunella vulgaris Linn as raw material, based on the research actuality of that, than did a series of work on Prunella vulgaris Linn .Firstly, polysaccharide from Prunella vulgaris Linn was extracted with water at 90℃. It was fractionated by anion-exchange chromatography and gel filtration chromatography. The results showed that weight average molecular weight of the major portion PLS3 was 8.3×10-5 Da by HPGPC. The primary structure of PLS3 was determined from its IR spectrum.Secondly, traditional phenol-vitriol method was amended to be more effective .In this paper, the manner of adding vitriol and the displaying temperature after adding vitriol effect on sensitive and absorbency Mixed standard samples were prepared based on the monosaccharide compositions to detect the quality of Prunella vulgaris Linn polysaccharide and PLS3.The result were 81.62%and 85.33% and the relative standard error were 0.56%and 0.34% respectively.Amended method effectively minished error and enhanced sensitive.Thirdly, an ion-pair reverse phase high performance liquid chromatographic (RP-HPLC)method for the simultaneous determination of carbohydrate and uronic acids was presented .p-Aminobenzoic acid(p-AMBA) was used for pre-column derivatization of the analytes ,enabling flurouescence (λex=313nm,λem=358nm)or photometric detection ( 303nm ) . Reaction condition such as reaction temperature and reaction time was optimized.Chromatographic column suitable for derivatization was selected. Effects of mobile phase compositions such as ion pairs and their concentrations and pH on the retention behaviors and separation results of 10 monosaccharides and 2 uronic were examzined. Derivatives of frucose, galactose, glucose, mannnose, xylose, arabose, ribose, N-acetyl-glucosamine, galacturonic acid, fucose, glucuronic acid , and rhamnose were separated on Atlantis dC18 column with hydrophilic end capping within 45 min ,applying sodium cyanoborohydride (TBAHSO4) as the ion pair reagent . The concentration detection limits was between 3.38 and 176×10-8 mol/L for fluorescence detection and between 2.55 and 13.4×10-7mol/L for UV detection. A good linearity was achieved in the concentration from 40 to143 mg/mL(R2>0.99). The changes of peak areas of derivative in 12-51 hours after derivatization were studied,and the RSD was from 2.5% to 3.9%. The described method has been applied for the determination of mono-/disaccharides and uronic acid in Prunella vulgaris Linn polysaccharide and spirulina polysaccharide after dissolved in trifluoroacetic acid solution (2 mol/L).And the result was nearly identical to that of ion chromatography and gas chromatography.After above research, we got better method to detect monosaccharide compositions of Prunella vulgaris Linn polysaccharide, discuss the detection and spectroscopy manner of Prunella vulgaris Linn polysaccharide.And these will benefit to food and medicine quality standard, offer useful gist to analysis on Prunella vulgaris Linn. And we look forward to making beneficial contribute to the deep exploitation on Prunella vulgaris Linn resource.

Related Dissertations

  1. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  2. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  3. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  4. Research of Question Answering System Based on the Analysis of Lexical and Semantic Meanings,TP391.1
  5. Research on Feature Extraction and Classification of Pulse Waveform for Cholecystitis and Nephrotic Syndrome Diagnosis,TP391.41
  6. Application of Q-Learning in the Content-Based Image Retrieval Technology,TP391.41
  7. Answer Extraction Based on Logic Representation and Reasoning for Reading Comprehension,TP391.1
  8. Research on Domain Entity Attribute and Event Extraction Technology,TP391.1
  9. Research on Transductive Support Vector Machine and Its Application in Image Retrieval,TP391.41
  10. Research on Feature Extraction and Classification of Tongue Shape and Tooth-Marked Tongue in TCM Tongue Diagnosis,TP391.41
  11. Research on Temporal Information Recognition and Normalization,TP391.1
  12. Context-Dependent Lexical Paraphrasing,TP391.1
  13. Research on Extraction and Tracking of People’s Opinion,TP391.1
  14. The Study of Domain Dictionary Construction Based on Web,TP391.1
  15. Research on Visual Measurement for Spacecraft Rendezvous and Approach,TP391.41
  16. Research on the Image Real-Time Acquisition, Storage and Image Processing System,TP391.41
  17. Feature Extraction, Selection and Combination in Lipreading,TP391.41
  18. Multi-currency Notes Technology Research and Implementation,TP391.41
  19. The Research on Paper Currency Classification Method Based on Harr-Like Feature and Minimal Ball Including Samples,TP391.41
  20. Pavement Distress Recognition Based on Image,TP391.41
  21. Research on Visual Detection and Tracking of Mobile Robots,TP242.62

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
© 2012 www.DissertationTopic.Net  Mobile