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Since 1993, Belgian scholars have reported that patients with progressive renal damage after taking Chinese herbal medicine containing fangchi and Magnolia weight loss treatment induced nephrotoxicity containing aristolochic acid components, this is defined as \acid nephropathy \Has more than ten years, during which many clinical studies and experimental studies have reported about the of AAN clinical features, pathological features and mechanism of the study. In recent years, research on the prevention and treatment of aristolochic acid nephropathy deepening, Aristolochia plants fangchi genus, the large number of domestic and international literature studied from different angles fangchi renal toxicity and its control. How to make a correct understanding of the validity of the compatibility of Chinese medicine, scientific scholars, in-depth study of traditional Chinese medicine detoxification synergies, and therefore proposed a study of this topic ideas, from the micro-level study the effect of acute renal toxicity of fangchi reversal effect . Purpose: This project from the cellular level research Astragalus fangchi acute renal toxicity reversal effect, and according to the astragalus and wide anti has different dose, Different Proportion, observed kidney biochemical indicators and pathological indicators, so as to optimize elected astragalus and wide anti-has the The best compatibility proportion and kidney toxicity alleviate fangchi thus confirming the theory of Chinese medicine is used in clinical the important and rationality of the principle of compatibility. Method: 1, select healthy female wister of rats, purchase collected from the Guangdong Zhaoqing Medicinal Herbs fangchi of Astragalus Shanxi Hunyuan County. 2 the single fried fangchi, Astragalus single fried, astragalus and wide Menispermaceae decoction at a set ratio, respectively, filtration, concentration, drying, crushing spare. 3, the experimental animals were randomly divided into control group fangchi own model group I (10g/kg), fangchi own model group II (20g/kg) fangchi (10g/kg) Astragalus decoction group is divided into (5 : 5; 5:4; 5:2), fangchi (20g/kg) and Astragalus decoction group is divided into (5:5; 5:4; 5:2), fangchi (10g/kg) Astragalus single the fried group is divided into (5:5,5:4,5:2), the fangchi (20g/kg) Astragalus single fried group is divided into (5:5,5:4,5:2), astragalus group (20g / kg) of 16 groups, each group gavage twice, experiments carried out 14 days after the determination of urinary protein, urine sugar, urine occult blood and urine NAG enzymes and urine β 2 -MG, were killed renal tissue of rats, blood to send biochemical tests and pathological examination of all the data for statistical analysis. The results: 1, observed rat behavior and body weight change display: fangchi group rats general condition and body weight compared with Astragalus fangchi group difference, there are significant with difference (P <0.05) fangchi renal toxicity, Astragalus plus wide anti-already renal toxicity with varying degrees of ease. 2, each rat urine comparison shows: fangchi group the urine sugar, urine occult blood and kidney damage indicators, and with the the dose of fangchi increases above indicators of change is more pronounced, Astragalus fangchi compatibility group varying degrees of urinary protein, urine sugar, urine occult blood mitigate and lightest group to the astragalus and fangchi (10g/kg) decoction (5:2), and close to normal, there is a significant difference (P <0.01). 3 rats renal: the group of fangchi urea nitrogen, serum creatinine, and more significantly increased with increasing doses of fangchi, the astragalus fangchi compatibility group urea nitrogen, serum creatinine liter The high degree of wider fangchi group light, and the proportion of different compatibility varying degrees of renal dysfunction, minimum set of values ??the astragalus fangchi (10g/kg) decoction (5:2), with a significant difference (P <0.01). 4 the the rats urine NAG enzymes and urine β 2 -MG Show: urinary NAG enzyme and β of fangchi group 2 -MG increased the most obvious, and with the compatibility groups higher dose also increased, compared with the control group, there is a significant difference, Astragalus fangchi, urine NAG enzymes β 2 -MG have varying degrees of change, the broader Menispermaceae group was significantly lower, which Astragalus fangchi (10g/kg) (5:2) group to change the most significant, with more significant difference (P <0.01). 5, each group of rat kidney tissue morphology comparison shows: between fangchi group renal interstitial edema, lymphocytic infiltration, tubular epithelial cell vacuolar degeneration, cytoplasmic shedding some tubular dilatation, glomerular and renal vascular no significant change, astragalus and fangchi compatibility group according to the ratio of compatibility of different, renal qualitative tubule damage degree of, but are wider fangchi group light, and there are significant differences, which astragalus and wide anti-already (10g/kg) (5 : 2) in renal tissue morphology close to normal, and each group has a significant difference (P <0.01). Conclusion: the single flavor fangchi kidney toxicity, similar pathological features with aristolochic acid nephropathy, and with dose doubling fangchi, increased renal toxicity. 2, the Astragalus fangchi compatibility lesions lighter Astragalus on acute renal toxicity fangchi varying degrees of reduced, suggesting that astragalus fangchi detoxification effect induced acute renal toxicity. 3, the Astragalus fangchi compatibility group, in order to the astragalus fangchi (10g/kg) at 5:2 ratio of mixed fried, detoxification effect is most obvious.
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